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Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69253 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69258 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Marker:Erik Jorgensen; Backbone Size:8941; Vector Backbone:pCFJ355; Vector Types:Worm Expression, Cre/Lox, MosSCI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26144318
Proper citation: RRID:Addgene_69256 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA(F+E) targeting pha-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_69488 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway cassette
Vector Backbone Description: Vector Backbone:pUCTCAPR3-GW; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30504365
Comments: bioRxiv doi.org/10.1101/445833
Proper citation: RRID:Addgene_74747 Copy
Species: Caenorhabditis elegans
Genetic Insert: guide RNA, flipped and extended version
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27506200
Comments: This vector is for cloning an sgRNA. Sequence is inserted as an annealed oligonucleotide linker into vector digested with BbsI.
Proper citation: RRID:Addgene_75026 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry::linker::H2B
Vector Backbone Description: Backbone Marker:Messing lab Plasmid 50005; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26187122
Proper citation: RRID:Addgene_70049 Copy
Species: Caenorhabditis elegans
Genetic Insert: TagRFP-T-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Comments: pDD286 is a derivative of our published TagRFP-SEC vector pDD284 (Dickinson et al. Genetics 2015, Addgene plasmid number 66825). It has a 3xMyc tag in place of 3xFlag, and Lox2272 sites in place of LoxP. These features allow pDD286 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites.
Proper citation: RRID:Addgene_70684 Copy
Species: Caenorhabditis elegans
Genetic Insert: mKate2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: pDD287is a derivative of our published TagRFP-SEC vector pDD285 (Dickinson et al. Genetics 2015, Addgene plasmid number 66825). It has a 3xMyc tag in place of 3xFlag, and Lox2272 sites in place of LoxP. These features allow pDD287 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites.
Proper citation: RRID:Addgene_70685 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65591 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APs12
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
Proper citation: RRID:Addgene_65599 Copy
Species: Caenorhabditis elegans
Genetic Insert: U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044730
Proper citation: RRID:Addgene_65630 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLG-1
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll ; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18255029
Comments: Mutation N142S does not affect plasmid function
Proper citation: RRID:Addgene_65828 Copy
Species: Caenorhabditis elegans
Genetic Insert: CoChR
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
CoChR amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66103 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Blelloch et al., 1999; Mathies et al., 2003; Vector Backbone:pJK590; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20110332
Proper citation: RRID:Addgene_71363 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16
Vector Backbone Description: Backbone Marker:Libina, et al. 2003. PMID 14622602; Vector Backbone:pNL205; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25960195
Proper citation: RRID:Addgene_71364 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1>mCherry::PH domain (PLC-delta)
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5712; Vector Backbone:pBS Sk-; Vector Types:bacterial propagation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19098902
Proper citation: RRID:Addgene_21743 Copy
Species: Caenorhabditis elegans
Genetic Insert: abu-1 promoter
Vector Backbone Description: Backbone Marker:Available at Addgene (#1494); Backbone Size:4487; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12186849
Comments: Addgene sequencing results show several mismatches with author's sequence.
abu-1::gfp C. elegans reporter gene. A 1.3-kb fragment of C. elegans genomic DNA immediately 5' of the predicted initiation ATG codon of abu-1 (AC3.3) was amplified by PCR using the oligonucleotides AC3.1S (5'-GGCATTGTGGCACGCATTGAACTG-3') and AC3Bam.2AS (5'-GATAGGATCCATTGTTAATATGCTTGAAGAGCTGC-3') and ligated in frame with the GFP coding region in the plasmid of pPD95.75.
Proper citation: RRID:Addgene_21824 Copy
Species: Caenorhabditis elegans
Genetic Insert: abu-1 promoter and coding region
Vector Backbone Description: Backbone Marker:Available at Addgene (#1494); Backbone Size:4487; Vector Backbone:pPD95_75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12186849
Comments: Addgene sequencing results show there is a S408P substitution in amino acid sequence in the coding region.
abu-1::abu-1::gfp C. elegans reporter gene. The promoter and coding region of abu-1 was ligated in frame with GFP to produce abu1::abu-1::gfp
Proper citation: RRID:Addgene_21825 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:16320; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16499902
Comments: Use ONLY DB3.1 to transform this plasmid. DH5 alpha does not work (it has a death gene).
Proper citation: RRID:Addgene_22720 Copy
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