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Species: Rattus norvegicus
Genetic Insert: dominant negative Rheb (D60K)
Vector Backbone Description: Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The published dominant negative D60K mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W by site directed mutagenesis. Although reported to have robust DN activity in other cell types, this construct did not provide robust DN activity in neurons.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32522 Copy
Species: Rattus norvegicus
Genetic Insert: Dominant negative Rheb (D60I)
Vector Backbone Description: Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The published dominant negative D60I mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W by site directed mutagenesis. Although reported to have robust DN activity in other cell types, this construct did not provide robust DN activity in neurons.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32521 Copy
Species: Rattus norvegicus
Genetic Insert: Rheb
Vector Backbone Description: Backbone Size:8450; Vector Backbone:pHAGE-CMV-eGFP-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20062052
Comments: Cloning of Rheb into the NotI and BamHI sites of pHAGE-CMV-eGFP-IRES-eGFP-W replaces the first copy of eGFP.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32519 Copy
Species: Rattus norvegicus
Genetic Insert: TRKB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_32500 Copy
Species: Rattus norvegicus
Genetic Insert: Dynamin I
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12011079
Proper citation: RRID:Addgene_32695 Copy
Species: Rattus norvegicus
Genetic Insert: Dynamin I
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12011079
Proper citation: RRID:Addgene_32696 Copy
Species: Rattus norvegicus
Genetic Insert: Mef2d
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:3176; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16484497
Comments: Can be used in conjunction with pcDNA3-rMEF2D (Addgene #32902) which is mutated so as to not be targeted by this shRNA.
Proper citation: RRID:Addgene_32900 Copy
Species: Rattus norvegicus
Genetic Insert: β-arrestin 1 S412A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Comments: Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing.
Proper citation: RRID:Addgene_42195 Copy
Species: Rattus norvegicus
Genetic Insert: Gamma 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22538369
Proper citation: RRID:Addgene_42200 Copy
Species: Rattus norvegicus
Genetic Insert: LAP-NLG1
Vector Backbone Description: Backbone Size:6389; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23457442
Proper citation: RRID:Addgene_43924 Copy
Species: Rattus norvegicus
Genetic Insert: CD200
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Comments: This plasmid contains an intentional G2A mutation in the CD200 sequence to create a consensus Kozak sequence
Proper citation: RRID:Addgene_36152 Copy
Species: Rattus norvegicus
Genetic Insert: G protein alpha 0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15078878
Proper citation: RRID:Addgene_36396 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin1
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17513300
Proper citation: RRID:Addgene_36918 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin2
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15501822
Proper citation: RRID:Addgene_36919 Copy
Species: Rattus norvegicus
Genetic Insert: SypHluorin 2x
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19217377
Proper citation: RRID:Addgene_37004 Copy
Species: Rattus norvegicus
Genetic Insert: SypHluorin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19217377
Proper citation: RRID:Addgene_37003 Copy
Species: Rattus norvegicus
Genetic Insert: ERK2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10521408
Proper citation: RRID:Addgene_37145 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9258; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_131483 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9263; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_131478 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9261; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_131476 Copy
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