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Species: Caenorhabditis elegans
Genetic Insert: pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159816 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159815 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159810 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159811 Copy
Species: Caenorhabditis elegans
Genetic Insert: [4-1] - Promoter - Pmlc-2(no_start)
Vector Backbone Description: Vector Backbone:pDONR P4-P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32908967
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159889 Copy
Species: Caenorhabditis elegans
Genetic Insert: [4-1] - Promoter - Psmu-2 (no_start)
Vector Backbone Description: Vector Backbone:pDONR P4-P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159887 Copy
Species: Caenorhabditis elegans
Genetic Insert: intron GG3 - 250bp no PATC
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159885 Copy
Species: Caenorhabditis elegans
Genetic Insert: [4-1] - Promoter - Psmu-1(no_atg)
Vector Backbone Description: Vector Backbone:pDONR P4-P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159886 Copy
Species: Caenorhabditis elegans
Genetic Insert: Intron GG1 - 900 bp
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159880 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
Proper citation: RRID:Addgene_159898 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmlc-1 | tagRFP | cbr-tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
Proper citation: RRID:Addgene_159899 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmlc-1 | gfp | cbr-tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
Proper citation: RRID:Addgene_159897 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
Proper citation: RRID:Addgene_159895 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3::wrmScarlet::tbb-2 UTR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: Protocols, sequence information, and updates can be found at: https://github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154824 Copy
Species: Caenorhabditis elegans
Genetic Insert: synthetic guide::∆HYGR::unc-54 3' UTR
Vector Backbone Description: Backbone Size:10683; Vector Backbone:pMS4; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: Additional protocol information can be found at: github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154838 Copy
Species: Caenorhabditis elegans
Genetic Insert: homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
Vector Backbone Description: Backbone Size:2470; Vector Backbone:pUC57; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: The LoxP site has a point mutation that in our hands does not noticeably impact function.
The rpl-28p::mKate2::unc-54 3'UTR transgene is flanked by restriction sites to facilitate removal and replacement of this gene.
Additional protocol information can be found at: github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154840 Copy
Species: Caenorhabditis elegans
Genetic Insert: Unc104 (1-653)
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pET17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12351789
Proper citation: RRID:Addgene_14107 Copy
Species: Caenorhabditis elegans
Genetic Insert: systemic RNA interference-deficient 1
Vector Backbone Description: Backbone Marker:A. Fire; Backbone Size:4000; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20512143
Comments: Specific details about this vector are given at the Materials and Methods of the article: Calixto et al, 2010, Nat Meth "Enhanced Neuronal RNAi using sid-1"
Note that this plasmid contains genomic sid-1, NOT the cDNA.
Proper citation: RRID:Addgene_25811 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Comments: extremely slow growing.
Proper citation: RRID:Addgene_26390 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26386 Copy
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