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Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153241 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153243 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153246 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153245 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153254 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153259 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153251 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153265 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153264 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153269 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153267 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153260 Copy
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153275 Copy
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153273 Copy
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Diatom cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: tet resistance cassette removed; ccdb resistance with att sites added
Proper citation: RRID:Addgene_154031 Copy
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Diatom cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: tet resistance cassette removed; ccdb resistance with att sites added; selectable marker added for centric diatoms
Proper citation: RRID:Addgene_154032 Copy
Vector Backbone Description: Backbone Size:7412; Vector Backbone:pMH-Halo tag; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_154144 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17574021
Comments: pWZL-Neo-Myr-Flag-DEST (pWN-MF-DEST) is a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF.
Created by inserting an N-terminal myr-FLAG tag and the Gateway-selectable cassette into the pWzl-Neo retroviral vector. To create specific pWN-MF-ORF vectors, perform site-specific LR recombination reactions.
LR reaction:
150ng DEST vector + 50ng ENTRY vector + 10xLR Buffer + LR enzyme + dH20. Incubate at 25oC for 1 hour. Add 1uL Proteinase K (20mg/ml), and incubate at 37oC for 10min. Transform into DH5alpha cells. See Invitrogen's website for detailed protocols and for LR enzyme.
Proper citation: RRID:Addgene_15300 Copy
Vector Backbone Description: Backbone Marker:Addgene Plasmid #49386; Backbone Size:6198; Vector Backbone:pcDNA3 APEX2-NES; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29625033
Proper citation: RRID:Addgene_149517 Copy
Vector Backbone Description: Vector Backbone:pLIX403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: 5' Cloning Site: PacI (not destroyed), 3' Cloning Site: SbfI (not destroyed)
Please note: Plasmid contains an IS1 insertion. This insertion is not expected to affect plasmid function as the sequence is not in a coding region of the plasmid and is outside of the lentiviral LTRs.
Proper citation: RRID:Addgene_158558 Copy
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