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Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 region
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Unpublished MosSCI reagents from the Jorgensen lab.
This vector targets the site ttTi4348 on Chr. I.
Use with strain EG6701 at the CGC.
Note - plasmid contains TWO M13F primer sites.
Proper citation: RRID:Addgene_30538 Copy
Genetic Insert: cat, sacB
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTP809; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10767554
Comments: pKM154 is a derivative of pBR322 that had sequence between the EcoR1 site and NdeI site removed, with a NotI site inserted (pTP809).
Proper citation: RRID:Addgene_13036 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5778; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13071 Copy
Genetic Insert: 6x Myc Tag
Vector Backbone Description: Backbone Size:5818; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13070 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:10365; Vector Backbone:pGAN049; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is one of the empty dual yTRAP sensor plasmids. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK247. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with pSK260.
Proper citation: RRID:Addgene_131148 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:11381; Vector Backbone:pGAN147; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty reverse yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN230. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin. To achieve dual color reporting, combine with pSK221.
Proper citation: RRID:Addgene_131122 Copy
Vector Backbone Description: Backbone Marker:Szilvia Kiriakov; Backbone Size:10377; Vector Backbone:pSK247; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty dual yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK234. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with an expression clone resulting from LR reaction using pGAN147 basic yTRAP sensor.
Proper citation: RRID:Addgene_131149 Copy
Species: Synthetic
Genetic Insert: PGK-puroR-pA cassette, inserted in PB-TET vector
Vector Backbone Description: Backbone Marker:Andras Nagy (Addgene plasmid # 20909); Backbone Size:13983; Vector Backbone:PB-TET (addgene #20909); Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_139561 Copy
Vector Backbone Description: Backbone Marker:Linzhao Cheng (Addgene plasmid # 28213); Backbone Size:9114; Vector Backbone:pEB-C5 (Addgene #28213); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone
Proper citation: RRID:Addgene_139519 Copy
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pCAGGS; Vector Types:Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted between loxP
Proper citation: RRID:Addgene_139517 Copy
Vector Backbone Description: Backbone Marker:Linzhao Cheng (Addgene plasmid # 28213); Backbone Size:11319; Vector Backbone:pEB-C5 (Addgene #28213); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone
Proper citation: RRID:Addgene_139520 Copy
Vector Backbone Description: Backbone Size:4338; Vector Backbone:pMC1DT-ApA; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone; Uses Diphtheria toxin A (DTA) as negative selection.
Proper citation: RRID:Addgene_139521 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
Vector Backbone Description: Backbone Size:17037; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: Second Generation pie-1 vector for expressing ORFs in the germline
Proper citation: RRID:Addgene_26955 Copy
Genetic Insert: pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:12296; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: For expressing GFP-ORF fusions in the germline. This vector does not contain unc-119 sequences and therefore must be co-injected with a marker (typically we use pRF4 in complex array injections - Kelly et al., 1997)
Proper citation: RRID:Addgene_26952 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:E.coli bacterial strain; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19633652
Comments: MG1655, bla, bio-, lambda-Red1, mutS–, cmR
From the paper: mutS in EcNR1 was replaced with the chloramphenicol resistance gene (cmR cassette).
Proper citation: RRID:Addgene_26931 Copy
Genetic Insert: pUC/ori, bla
Vector Backbone Description: Backbone Marker:Gateway(R) (Invitrogen); Backbone Size:3012; Vector Backbone:pDONRP2R-P3; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21203517
Proper citation: RRID:Addgene_27367 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
Vector Backbone Description: Backbone Size:6000; Vector Backbone:psh100; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33037152
Proper citation: RRID:Addgene_160934 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161880 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161883 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161878 Copy
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