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Species: Caenorhabditis elegans
Genetic Insert: Gateway(attR1-attR2)::FLP::let-858(3’)-(Cbr)unc-119(+)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC31; Vector Types:Worm Expression, FLP/FRT, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19616 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19629 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC91; Vector Types:Worm Expression, FLP/FRT, destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19628 Copy
Species: Caenorhabditis elegans
Genetic Insert:
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19672 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R4-R3)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19676 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19675 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19678 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R4-R3)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19677 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7624; Vector Backbone:pCDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19702 Copy
Genetic Insert: pgLAP3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8325; Vector Backbone:pEF5/FRT-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19704 Copy
Genetic Insert: pgLAP5
Vector Backbone Description: Backbone Size:8365; Vector Backbone:pEF5/FRT-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19706 Copy
Genetic Insert: pgLAP4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7638; Vector Backbone:pEF5/FRT-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_19705 Copy
Species: cloning vector
Genetic Insert: pWS-TK2
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18546598
Comments: The deposited sequence file of pWS-TK2 is from compilation of previous plasmids, and only part of it has been re-sequenced. There is a gap between author's sequence and Addgene sequence that will NOT affect the use of this plasmid.
Please also note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.
pWS-TK2 is designed to have two variants of thymidine kinase gene from herpes virus.
Proper citation: RRID:Addgene_20348 Copy
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:9370; Vector Backbone:pBluescript KS; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19252478
Proper citation: RRID:Addgene_20909 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:E.coli bacterial strain; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19633652
Comments: MG1655, bla, bio-, lambda-Red1, mutS–, cmR
From the paper: mutS in EcNR1 was replaced with the chloramphenicol resistance gene (cmR cassette).
Proper citation: RRID:Addgene_26931 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
Vector Backbone Description: Backbone Size:17037; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: Second Generation pie-1 vector for expressing ORFs in the germline
Proper citation: RRID:Addgene_26955 Copy
Genetic Insert: pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:12296; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: For expressing GFP-ORF fusions in the germline. This vector does not contain unc-119 sequences and therefore must be co-injected with a marker (typically we use pRF4 in complex array injections - Kelly et al., 1997)
Proper citation: RRID:Addgene_26952 Copy
Genetic Insert: pUC/ori, bla
Vector Backbone Description: Backbone Marker:Gateway(R) (Invitrogen); Backbone Size:3012; Vector Backbone:pDONRP2R-P3; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21203517
Proper citation: RRID:Addgene_27367 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:16320; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16499902
Comments: Use ONLY DB3.1 to transform this plasmid. DH5 alpha does not work (it has a death gene).
Proper citation: RRID:Addgene_22720 Copy
Species: artifical
Genetic Insert: Gateway-SnAvi
Vector Backbone Description: Backbone Marker:pTriEx5 (Novagen); Backbone Size:6027; Vector Backbone:pBY2887; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20047968
Proper citation: RRID:Addgene_23222 Copy
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