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  • Bacterial Resistance:ampicillin and kanamycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
YSBE415: pTOP_ACA1_Vc_HOG1 terminator
 
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RRID:Addgene_122434 C-terminal fragment of Venus protein Other Ampicillin and Kanamycin PMID:27780958 Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:15:17 0
YSBE420:pNEO_CAC1_Vn_HOG1 terminator
 
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RRID:Addgene_122701 N-terminal fragment of Venus protein Cryptococcus neoformans Ampicillin and Kanamycin PMID:27780958 Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:15:21 0
YSBE418:pNAT_ACA1_Vc_HOG1 terminator
 
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RRID:Addgene_122700 C-terminal fragment of Venus protein Cryptococcus neoformans Ampicillin and Kanamycin PMID:27780958 Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:15:21 0
pGG431
 
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RRID:Addgene_165615 TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID) Ampicillin and Kanamycin PMID:33441553 Backbone Marker:Marcus Noyes Lab; Vector Backbone:pGG211; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin Corrected homology relative to wild type SpCas9 (immediately adjacent to the NheI site upstream of KanR cassette) 2026-09-01 09:27:03 0
pGG442
 
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RRID:Addgene_165616 TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t Ampicillin and Kanamycin PMID:33441553 Backbone Marker:Marcus Noyes Lab; Vector Backbone:pGG211; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin Corrected homology relative to wild type SpCas9 (immediately adjacent to the NheI site upstream of KanR cassette) and inserted Zif268 immediately upstream of the C-terminal NLS (with a linker fragment between SpCas9 and Zif268) 2026-09-01 09:27:03 0
T-B18
 
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RRID:Addgene_159446 BsMDH BmHPS BmPHI (on Addgene plasmid # 129104) Bacillus subtilis and Bacillus methanolicus (for plasmid gene insert) Ampicillin and Kanamycin PMID:33404828 This T-B18 E.coli strain harbors the pETM6_Ptrc_BsMdh_BmHps_BmPhi plasmid (Addgene plasmid # 129104), which enables the evolved E. coli strain to metabolize methanol in its central carbon metabolism. It was adaptively evolved from the parent strain, E. coli BW25113 ΔfrmA::kanR (obtained from the Keio collection) containing Addgene plasmid # 129104 such that it grows efficiently with just 2mM Thr and 250 mM MeOH. This strain has been functionally validated for growth by the depositing laboratory. Addgene has not confirmed the plasmid by QC sequence analysis. Backbone Marker:Eleftherios Terry Papoutsakis (Addgene plasmid # 129104); Vector Backbone:pETM6_Ptrc_BsMdh_BmHps_BmPhi; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin see Supplemental Documents for genome analysis of strain T-B18 against a reference BW25113 genome 2026-09-01 09:25:20 0
pCPP6534
 
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RRID:Addgene_128735 Ampicillin and Kanamycin PMID:29742421 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:17:08 0
pCR2.1-KRAS-G12V
 
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RRID:Addgene_129091 KRAS(G12V) Homo sapiens Ampicillin and Kanamycin PMID:25706875 Vector Backbone:pCR2.1-TOPO cloning vector; Vector Types:homologous recombination donor plasmid; Bacterial Resistance:Ampicillin and Kanamycin G12V 2026-09-01 09:17:14 0
p-sfGFP-pA-FRT-Amp-FRT
 
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RRID:Addgene_176620 super folder green fluorescent protein with FRT-flanked ampicillin cassette Synthetic Ampicillin and Kanamycin PMID:34751773 Please note depositor confirms H217R in insert does not affect plasmid function. Backbone Marker:Clontech; Backbone Size:3700; Vector Backbone:pEGFP-C1; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin H217R - please see dep. comments 2026-09-01 09:30:00 0
pIKM1
 
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RRID:Addgene_51054 Ampicillin and Kanamycin PMID:14766568 For more information about this plasmid, see the associated publication (PMID: 14766568), as well as the publication describing its original construction: Mai, Lorenz, and Wiegel, Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance. FEMS Microbiol Lett. 1997 DOI: 10.1111/j.1574-6968.1997.tb10283.x ( http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1997.tb10283.x/abstract ) Backbone Marker:Mermelstein et al. 1992; Backbone Size:4500; Vector Backbone:pIMP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:56:27 0
p2attNG-H11-short
 
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1+ mentions
RRID:Addgene_51546 GFP4m Ampicillin and Kanamycin PMID:24304893 Vector Backbone:p2attNG; Vector Types:Mammalian Expression, Human Targeting; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:56:33 1
pCSCX
 
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RRID:Addgene_63918 cscAKB Escherichia coli W Ampicillin and Kanamycin PMID:21907272 The transferrable sucrose utilization cassette contains cscA (invertase), cscK (d-fructokinase), and cscB (sucrose permease). Backbone Marker:Life Technologies; Backbone Size:4059; Vector Backbone:pCR2.1; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:59:11 0
pSEVA12DKR-J
 
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RRID:Addgene_58306 Genomic cassette Synthetic Ampicillin and Kanamycin PMID:24847673 Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:57:59 0
pSEVA12TEC-I
 
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RRID:Addgene_58308 Genomic cassette Synthetic Ampicillin and Kanamycin PMID:24847673 Please note that Addgene's NGS found a 1209bp transposase insertion between the RepA and Epsilon Antitoxin sequence. The effect of this insertion is unknown. If you are interested in the version without the insertion, please contact the depositing lab (Tom Ellis lab). Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:57:59 0
pZmU3-gRNA
 
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1+ mentions
RRID:Addgene_53061 zmU3 promoter - gRNA maize (corn) Ampicillin and Kanamycin PMID:24576457 Backbone Marker:Transgen; Backbone Size:3929; Vector Backbone:pEasy-blunt; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:56:51 1
mCherryS131C
 
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RRID:Addgene_48732 mCherryS131C Synthetic Ampicillin and Kanamycin PMID:21696135 This plasmid must be grown under Ampicillin (100ug/ml) and Kanamycin (50ug/ml) selection in order to retain both the mCherryS131C plasmid and the pREP4 helper plasmid. Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results for recommended primers. The S131C mutation in this plasmid is S136C, when compared to the starting Met of wild-type mCherry. Backbone Marker:QIAGEN; Backbone Size:3439; Vector Backbone:pQE-9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin changed serine 131 to cysteine 2026-09-01 09:56:00 0
pTOPO-rDNA-NTS
 
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RRID:Addgene_23992 rDNA NTS Saccharomyces cerevisiae Ampicillin and Kanamycin PMID:15489292 Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin None 2026-09-01 09:47:12 0
pCRII Topo-Tbx20 (14)
 
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1+ mentions
RRID:Addgene_24715 Tbx20 Mus musculus Ampicillin and Kanamycin Mouse Tbx20 from e12.5 mouse heart cDNA was isolated by PCR. Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:48:56 2
ptia1l-hsp-GFP
 
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RRID:Addgene_177837 tia1l-sgRNA target site-hsp70-EGFP Danio rerio Ampicillin and Kanamycin PMID:36050398 The tia1l sgRNA was designed and published by Hwang et al., 2013, Nature Biotechnology: https://pubmed.ncbi.nlm.nih.gov/23360964/. The strategy is based on a method described by Lackner et al. 2015, Nature Communications: https://pubmed.ncbi.nlm.nih.gov/26674669/. Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1-TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 09:30:23 0
YSBE 557 : pNAT_GFP
 
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RRID:Addgene_92085 GFP Other Ampicillin and Kanamycin PMID:27780958 Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 10:05:24 0

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