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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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YSBE415: pTOP_ACA1_Vc_HOG1 terminator Resource Report Resource Website |
RRID:Addgene_122434 | C-terminal fragment of Venus protein | Other | Ampicillin and Kanamycin | PMID:27780958 | Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:15:17 | 0 | ||
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YSBE420:pNEO_CAC1_Vn_HOG1 terminator Resource Report Resource Website |
RRID:Addgene_122701 | N-terminal fragment of Venus protein | Cryptococcus neoformans | Ampicillin and Kanamycin | PMID:27780958 | Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:15:21 | 0 | ||
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YSBE418:pNAT_ACA1_Vc_HOG1 terminator Resource Report Resource Website |
RRID:Addgene_122700 | C-terminal fragment of Venus protein | Cryptococcus neoformans | Ampicillin and Kanamycin | PMID:27780958 | Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:15:21 | 0 | ||
|
pGG431 Resource Report Resource Website |
RRID:Addgene_165615 | TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID) | Ampicillin and Kanamycin | PMID:33441553 | Backbone Marker:Marcus Noyes Lab; Vector Backbone:pGG211; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin | Corrected homology relative to wild type SpCas9 (immediately adjacent to the NheI site upstream of KanR cassette) | 2026-09-01 09:27:03 | 0 | ||
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pGG442 Resource Report Resource Website |
RRID:Addgene_165616 | TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t | Ampicillin and Kanamycin | PMID:33441553 | Backbone Marker:Marcus Noyes Lab; Vector Backbone:pGG211; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin | Corrected homology relative to wild type SpCas9 (immediately adjacent to the NheI site upstream of KanR cassette) and inserted Zif268 immediately upstream of the C-terminal NLS (with a linker fragment between SpCas9 and Zif268) | 2026-09-01 09:27:03 | 0 | ||
|
T-B18 Resource Report Resource Website |
RRID:Addgene_159446 | BsMDH BmHPS BmPHI (on Addgene plasmid # 129104) | Bacillus subtilis and Bacillus methanolicus (for plasmid gene insert) | Ampicillin and Kanamycin | PMID:33404828 | This T-B18 E.coli strain harbors the pETM6_Ptrc_BsMdh_BmHps_BmPhi plasmid (Addgene plasmid # 129104), which enables the evolved E. coli strain to metabolize methanol in its central carbon metabolism. It was adaptively evolved from the parent strain, E. coli BW25113 ΔfrmA::kanR (obtained from the Keio collection) containing Addgene plasmid # 129104 such that it grows efficiently with just 2mM Thr and 250 mM MeOH. This strain has been functionally validated for growth by the depositing laboratory. Addgene has not confirmed the plasmid by QC sequence analysis. | Backbone Marker:Eleftherios Terry Papoutsakis (Addgene plasmid # 129104); Vector Backbone:pETM6_Ptrc_BsMdh_BmHps_BmPhi; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | see Supplemental Documents for genome analysis of strain T-B18 against a reference BW25113 genome | 2026-09-01 09:25:20 | 0 |
|
pCPP6534 Resource Report Resource Website |
RRID:Addgene_128735 | Ampicillin and Kanamycin | PMID:29742421 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:17:08 | 0 | |||
|
pCR2.1-KRAS-G12V Resource Report Resource Website |
RRID:Addgene_129091 | KRAS(G12V) | Homo sapiens | Ampicillin and Kanamycin | PMID:25706875 | Vector Backbone:pCR2.1-TOPO cloning vector; Vector Types:homologous recombination donor plasmid; Bacterial Resistance:Ampicillin and Kanamycin | G12V | 2026-09-01 09:17:14 | 0 | |
|
p-sfGFP-pA-FRT-Amp-FRT Resource Report Resource Website |
RRID:Addgene_176620 | super folder green fluorescent protein with FRT-flanked ampicillin cassette | Synthetic | Ampicillin and Kanamycin | PMID:34751773 | Please note depositor confirms H217R in insert does not affect plasmid function. | Backbone Marker:Clontech; Backbone Size:3700; Vector Backbone:pEGFP-C1; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin | H217R - please see dep. comments | 2026-09-01 09:30:00 | 0 |
|
pIKM1 Resource Report Resource Website |
RRID:Addgene_51054 | Ampicillin and Kanamycin | PMID:14766568 | For more information about this plasmid, see the associated publication (PMID: 14766568), as well as the publication describing its original construction: Mai, Lorenz, and Wiegel, Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance. FEMS Microbiol Lett. 1997 DOI: 10.1111/j.1574-6968.1997.tb10283.x ( http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1997.tb10283.x/abstract ) | Backbone Marker:Mermelstein et al. 1992; Backbone Size:4500; Vector Backbone:pIMP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:56:27 | 0 | |||
|
p2attNG-H11-short Resource Report Resource Website 1+ mentions |
RRID:Addgene_51546 | GFP4m | Ampicillin and Kanamycin | PMID:24304893 | Vector Backbone:p2attNG; Vector Types:Mammalian Expression, Human Targeting; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:56:33 | 1 | |||
|
pCSCX Resource Report Resource Website |
RRID:Addgene_63918 | cscAKB | Escherichia coli W | Ampicillin and Kanamycin | PMID:21907272 | The transferrable sucrose utilization cassette contains cscA (invertase), cscK (d-fructokinase), and cscB (sucrose permease). | Backbone Marker:Life Technologies; Backbone Size:4059; Vector Backbone:pCR2.1; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:59:11 | 0 | |
|
pSEVA12DKR-J Resource Report Resource Website |
RRID:Addgene_58306 | Genomic cassette | Synthetic | Ampicillin and Kanamycin | PMID:24847673 | Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:57:59 | 0 | ||
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pSEVA12TEC-I Resource Report Resource Website |
RRID:Addgene_58308 | Genomic cassette | Synthetic | Ampicillin and Kanamycin | PMID:24847673 | Please note that Addgene's NGS found a 1209bp transposase insertion between the RepA and Epsilon Antitoxin sequence. The effect of this insertion is unknown. If you are interested in the version without the insertion, please contact the depositing lab (Tom Ellis lab). | Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:57:59 | 0 | |
|
pZmU3-gRNA Resource Report Resource Website 1+ mentions |
RRID:Addgene_53061 | zmU3 promoter - gRNA | maize (corn) | Ampicillin and Kanamycin | PMID:24576457 | Backbone Marker:Transgen; Backbone Size:3929; Vector Backbone:pEasy-blunt; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:56:51 | 1 | ||
|
mCherryS131C Resource Report Resource Website |
RRID:Addgene_48732 | mCherryS131C | Synthetic | Ampicillin and Kanamycin | PMID:21696135 | This plasmid must be grown under Ampicillin (100ug/ml) and Kanamycin (50ug/ml) selection in order to retain both the mCherryS131C plasmid and the pREP4 helper plasmid. Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results for recommended primers. The S131C mutation in this plasmid is S136C, when compared to the starting Met of wild-type mCherry. | Backbone Marker:QIAGEN; Backbone Size:3439; Vector Backbone:pQE-9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | changed serine 131 to cysteine | 2026-09-01 09:56:00 | 0 |
|
pTOPO-rDNA-NTS Resource Report Resource Website |
RRID:Addgene_23992 | rDNA NTS | Saccharomyces cerevisiae | Ampicillin and Kanamycin | PMID:15489292 | Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin | None | 2026-09-01 09:47:12 | 0 | |
|
pCRII Topo-Tbx20 (14) Resource Report Resource Website 1+ mentions |
RRID:Addgene_24715 | Tbx20 | Mus musculus | Ampicillin and Kanamycin | Mouse Tbx20 from e12.5 mouse heart cDNA was isolated by PCR. | Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:48:56 | 2 | ||
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ptia1l-hsp-GFP Resource Report Resource Website |
RRID:Addgene_177837 | tia1l-sgRNA target site-hsp70-EGFP | Danio rerio | Ampicillin and Kanamycin | PMID:36050398 | The tia1l sgRNA was designed and published by Hwang et al., 2013, Nature Biotechnology: https://pubmed.ncbi.nlm.nih.gov/23360964/. The strategy is based on a method described by Lackner et al. 2015, Nature Communications: https://pubmed.ncbi.nlm.nih.gov/26674669/. | Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1-TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 09:30:23 | 0 | |
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YSBE 557 : pNAT_GFP Resource Report Resource Website |
RRID:Addgene_92085 | GFP | Other | Ampicillin and Kanamycin | PMID:27780958 | Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin | 2026-09-01 10:05:24 | 0 |
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