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Species: Other
Genetic Insert: C-terminal fragment of Venus protein
Vector Backbone Description: Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958
Proper citation: RRID:Addgene_122434 Copy
Species: Cryptococcus neoformans
Genetic Insert: N-terminal fragment of Venus protein
Vector Backbone Description: Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958
Proper citation: RRID:Addgene_122701 Copy
Species: Cryptococcus neoformans
Genetic Insert: C-terminal fragment of Venus protein
Vector Backbone Description: Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958
Proper citation: RRID:Addgene_122700 Copy
Genetic Insert: TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab; Vector Backbone:pGG211; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:33441553
Proper citation: RRID:Addgene_165615 Copy
Genetic Insert: TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab; Vector Backbone:pGG211; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:33441553
Proper citation: RRID:Addgene_165616 Copy
Species: Bacillus subtilis and Bacillus methanolicus (for plasmid gene insert)
Genetic Insert: BsMDH BmHPS BmPHI (on Addgene plasmid # 129104)
Vector Backbone Description: Backbone Marker:Eleftherios Terry Papoutsakis (Addgene plasmid # 129104); Vector Backbone:pETM6_Ptrc_BsMdh_BmHps_BmPhi; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:33404828
Comments: This T-B18 E.coli strain harbors the pETM6_Ptrc_BsMdh_BmHps_BmPhi plasmid (Addgene plasmid # 129104), which enables the evolved E. coli strain to metabolize methanol in its central carbon metabolism. It was adaptively evolved from the parent strain, E. coli BW25113 ΔfrmA::kanR (obtained from the Keio collection) containing Addgene plasmid # 129104 such that it grows efficiently with just 2mM Thr and 250 mM MeOH.
This strain has been functionally validated for growth by the depositing laboratory. Addgene has not confirmed the plasmid by QC sequence analysis.
Proper citation: RRID:Addgene_159446 Copy
Vector Backbone Description: Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29742421
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_128735 Copy
Species: Homo sapiens
Genetic Insert: KRAS(G12V)
Vector Backbone Description: Vector Backbone:pCR2.1-TOPO cloning vector; Vector Types:homologous recombination donor plasmid; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:25706875
Proper citation: RRID:Addgene_129091 Copy
Species: Synthetic
Genetic Insert: super folder green fluorescent protein with FRT-flanked ampicillin cassette
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3700; Vector Backbone:pEGFP-C1; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34751773
Comments: Please note depositor confirms H217R in insert does not affect plasmid function.
Proper citation: RRID:Addgene_176620 Copy
Vector Backbone Description: Backbone Marker:Mermelstein et al. 1992; Backbone Size:4500; Vector Backbone:pIMP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:14766568
Comments: For more information about this plasmid, see the associated publication (PMID: 14766568), as well as the publication describing its original construction: Mai, Lorenz, and Wiegel, Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance. FEMS Microbiol Lett. 1997 DOI: 10.1111/j.1574-6968.1997.tb10283.x ( http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1997.tb10283.x/abstract )
Proper citation: RRID:Addgene_51054 Copy
Genetic Insert: GFP4m
Vector Backbone Description: Vector Backbone:p2attNG; Vector Types:Mammalian Expression, Human Targeting; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24304893
Proper citation: RRID:Addgene_51546 Copy
Species: Escherichia coli W
Genetic Insert: cscAKB
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4059; Vector Backbone:pCR2.1; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21907272
Comments: The transferrable sucrose utilization cassette contains cscA (invertase), cscK (d-fructokinase), and cscB (sucrose permease).
Proper citation: RRID:Addgene_63918 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58306 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Comments: Please note that Addgene's NGS found a 1209bp transposase insertion between the RepA and Epsilon Antitoxin sequence. The effect of this insertion is unknown. If you are interested in the version without the insertion, please contact the depositing lab (Tom Ellis lab).
Proper citation: RRID:Addgene_58308 Copy
Species: maize (corn)
Genetic Insert: zmU3 promoter - gRNA
Vector Backbone Description: Backbone Marker:Transgen; Backbone Size:3929; Vector Backbone:pEasy-blunt; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24576457
Proper citation: RRID:Addgene_53061 Copy
Species: Synthetic
Genetic Insert: mCherryS131C
Vector Backbone Description: Backbone Marker:QIAGEN; Backbone Size:3439; Vector Backbone:pQE-9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21696135
Comments: This plasmid must be grown under Ampicillin (100ug/ml) and Kanamycin (50ug/ml) selection in order to retain both the mCherryS131C plasmid and the pREP4 helper plasmid.
Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results for recommended primers.
The S131C mutation in this plasmid is S136C, when compared to the starting Met of wild-type mCherry.
Proper citation: RRID:Addgene_48732 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rDNA NTS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23992 Copy
Species: Mus musculus
Genetic Insert: Tbx20
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Mouse Tbx20 from e12.5 mouse heart cDNA was isolated by PCR.
Proper citation: RRID:Addgene_24715 Copy
Species: Danio rerio
Genetic Insert: tia1l-sgRNA target site-hsp70-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1-TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36050398
Comments: The tia1l sgRNA was designed and published by Hwang et al., 2013, Nature Biotechnology: https://pubmed.ncbi.nlm.nih.gov/23360964/.
The strategy is based on a method described by Lackner et al. 2015, Nature Communications: https://pubmed.ncbi.nlm.nih.gov/26674669/.
Proper citation: RRID:Addgene_177837 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958
Proper citation: RRID:Addgene_92085 Copy
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