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  • Organism:caenorhabditis elegans (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pNM3811
 
Resource Report
Resource Website
RRID:Addgene_154120 mec-4p::QF2::act-4 '3 Caenorhabditis elegans Ampicillin PMID:32513816 Backbone Marker:Addgene #153083; Backbone Size:7855; Vector Backbone:pLF3FShC; Vector Types:Worm Expression, RMCE; Bacterial Resistance:Ampicillin 2026-09-01 09:23:53 0
pJG103
 
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RRID:Addgene_158533 wrmScarlet11 x3 tandem repeats Caenorhabditis elegans Kanamycin PMID:33693628 Please visit https://www.biorxiv.org/content/10.1101/2020.07.02.185249v1 for bioRxiv preprint. Backbone Marker:Genewiz; Backbone Size:2626; Vector Backbone:pUC57-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:25:06 0
pAS18
 
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RRID:Addgene_158787 Ptag-168::GCaMP6m::rpl-1a Caenorhabditis elegans Ampicillin PMID:32574560 Backbone Marker:C.I. Bargmann and S. MaCarroll; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:11 0
pHCMM4
 
Resource Report
Resource Website
RRID:Addgene_170359 PCYC1_SEC61_mCherry_LEU2_TCYC1 Caenorhabditis elegans Ampicillin PMID:32907940 Backbone Size:4872; Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:28:16 0
pAAP20
 
Resource Report
Resource Website
RRID:Addgene_171206 meg-3 Caenorhabditis elegans Kanamycin Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint. Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin aa545-862 2026-09-01 09:28:30 0
pHS33
 
Resource Report
Resource Website
RRID:Addgene_171202 meg-3 Caenorhabditis elegans Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint. Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa545-862, F705A,Y708A,Y713A, N725A 2026-09-01 09:28:30 0
pJW4.04
 
Resource Report
Resource Website
RRID:Addgene_171209 pgl-3 Caenorhabditis elegans Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint. Vector Backbone:pMAL-c2X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:28:30 0
pJK2020
 
Resource Report
Resource Website
RRID:Addgene_171932 Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR Caenorhabditis elegans Ampicillin PMID:31378588 Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:28:42 0
pJK2014
 
Resource Report
Resource Website
RRID:Addgene_171931 Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR Caenorhabditis elegans Ampicillin PMID:31378588 Vector Backbone:pCFJ151; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:28:42 0
pDD283
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_66824 YPET-C1^SEC^3xFlag Caenorhabditis elegans Ampicillin PMID:26044593 IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 09:59:48 3
pDD282
 
Resource Report
Resource Website
50+ mentions
RRID:Addgene_66823 GFP-C1^SEC^3xFlag Caenorhabditis elegans Ampicillin PMID:26044593 IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. Sequence note: The full sequence is based on Addgene NGS of our stock. There may be some slight but innocuous inconsistencies with the depositor's GenBank files. One discrepancy affects the sequence of the 3' arm reverse primer (Table P1 and Figure P1) from the associated publication. The first g from the 3' end is missing:[tcacacaggaaacagctatgaccatgttat] -> [tcacacaggaaacagctatgaccatttat] Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 09:59:48 51
pClneoEGFPC RSF-1
 
Resource Report
Resource Website
RRID:Addgene_66862 RSF-1 Caenorhabditis elegans Ampicillin PMID:23103556 Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S18G, S229T, I403T and F563S mutations in RSF-1 compared to GenBank reference sequence NP_001022361.1 2026-09-01 09:59:48 0
pClneoFH-let60 G12V
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_66860 Let-60 Caenorhabditis elegans Ampicillin PMID:23103556 Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Glycine 12 is mutated to valine 2026-09-01 09:59:48 1
pPD95.75-cYAP-1
 
Resource Report
Resource Website
RRID:Addgene_66857 C.elegans YAP-1 Caenorhabditis elegans Ampicillin PMID:23396260 I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine. Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin Serine104 is mutated to alanine 2026-09-01 09:59:48 0
pClneoMyc'-EGL-44
 
Resource Report
Resource Website
RRID:Addgene_66855 EGL-44 Caenorhabditis elegans Ampicillin PMID:23396260 Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:59:48 0
pFLAG-WTS-1
 
Resource Report
Resource Website
RRID:Addgene_66856 WTS-1 Caenorhabditis elegans Ampicillin PMID:23396260 Backbone Marker:Eastman Kodak Company; Backbone Size:4700; Vector Backbone:pFLAG-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E153G and D871G mutations in WTS1 compared to GenBank reference sequence NP_492699.1 2026-09-01 09:59:48 0
pKPY737
 
Resource Report
Resource Website
RRID:Addgene_72673 Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221 Caenorhabditis elegans Kanamycin Note: the first methionine in the alpha subunit of C. elegans PheRS, Isoform A, Exon 3 is designated as residue 1 (“Met1”). Backbone Marker:Invitrogen; Backbone Size:2544; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin Thr412Gly in CePheRS Alpha Subunit 2026-09-01 10:01:04 0
pKPY738
 
Resource Report
Resource Website
RRID:Addgene_72674 let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3 Caenorhabditis elegans Kanamycin Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-09-01 10:01:04 0
pDD315 (mTurquoise2^SEC^2xHA)
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_73343 mTurquoise2-C1^SEC^3xFlag Caenorhabditis elegans Ampicillin PMID:37351566 pDD315 is a derivative of our published vectors pDD282-285 (Dickinson et al. Genetics 2015). It has a 2xHA tag in place of 3xFlag, and Lox511I sites in place of LoxP. These features allow pDD315 to be used in a genetic background that has already been modified using a green or red FP-SEC vector, without conflicts between epitope tags and Lox sites. Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 10:01:13 2
pDD317 (Pmyo-2::GFP + SEC)
 
Resource Report
Resource Website
RRID:Addgene_73344 Pmyo-2::GFP + SEC Caenorhabditis elegans Ampicillin PMID:34903234 pDD317 is a derivative of our published vectors pDD282-285 (Dickinson et al. Genetics 2015) and is intended for making gene knockouts. It has a Pmyo-2::GFP expression cassette (bright pharyngeal fluorescence) in place of the simple fluorescent protein found in our other FP–SEC vectors. Pmyo-2::GFP + SEC is intended to be inserted in place of an entire ORF such that after insertion and SEC removal, the knockout is marked with Pmyo-2::GFP. The vector also has Lox511I sites in place of LoxP, allowing pDD315 to be used in a genetic background that has already been modified using a green or red FP-SEC vector, without conflicts between Lox sites. Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 10:01:13 0

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