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Genetic Insert: Tev Protease Cleavage site
Vector Backbone Description: Backbone Size:2720; Vector Backbone:pMLL-AC; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Note that this plasmid must be grown in both Ampicillin and Chloramphenicol
Proper citation: RRID:Addgene_26186 Copy
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for phleomycin selection of transgenes in C. elegans.
The PuroR gene in pBCN21-R4R3 was replaced by the ZeocinR gene for phleomycin resistance.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26305 Copy
Vector Backbone Description: Backbone Size:5879; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for puromycin selection of transgenes in C. elegans and related species if transgene has a visual phenotype and Pmyo-2::mCherry expression is undesirable.
The unc-119 gene in pCG150 was replaced by the Prpl-28::PuroR::let-858_3'UTR cassette for puromycin selection in worms.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26301 Copy
Vector Backbone Description: Backbone Size:5969; Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for G418 selection of transgenes in C. elegans.
The PuroR gene in pBCN21-R4R3 was replaced by the NeoR gene for G418 resistance.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26304 Copy
Species: Homo sapiens
Genetic Insert: GAL4::p65
Vector Backbone Description: Backbone Size:8778; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26229 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9052; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Comments: Addgene NGS results identified a second GAL4 ORF in the plasmid sequence. This second ORF is not expected to affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_26226 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:9409; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26236 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:9409; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26235 Copy
Species: Homo sapiens
Genetic Insert: LexA::p65
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26231 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Zip-GAL4DBD
Vector Backbone Description: Backbone Size:8731; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26233 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Comments: extremely slow growing.
Proper citation: RRID:Addgene_26390 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26386 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:10104; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced in a Tet-On advanced cell line in order to obtain expression of the GOI.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.
Addgene found this plasmid to be extremely slow growing.
Proper citation: RRID:Addgene_26433 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:9185; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced in a Tet-On Advanced cell line for expression of the GOI.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.
Proper citation: RRID:Addgene_26432 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5070; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21344536
Proper citation: RRID:Addgene_26361 Copy
Vector Backbone Description: Backbone Size:10809; Vector Backbone:pCJF150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: The Prpl-28::PuroR::let-858_3'UTR cassette was inserted into the backbone of pCFJ150 to create a puromycin-selectable MosSCI vector for single copy integration in worms at the ttTi5605.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26346 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182167 Copy
Species: Other
Genetic Insert: mTurquoise
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182169 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Please note: The plasmid contains an AT nucleotide deletion in the AT-repeat region of Mhc-F4-678-EGFP::GW. This deletion is not expected to have any functional consequences on the plasmid.
Proper citation: RRID:Addgene_182170 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:33000; Vector Backbone:AdenoX; Vector Types:Adenoviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21415370
Comments: He, A. and Pu, W. T. (2010) Genome-wide location analysis by pull down of in vivo biotinylated transcription factors. Curr Protoc Mol Biol Chapter 21, Unit 21.20
Please note that Addgene's sequencing results differ from the sequence provided by the depositing scientist at nucleotides 1778 and 1996. This mismatches are not known to affect plasmid function.
Proper citation: RRID:Addgene_30217 Copy
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