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Species: Synthetic
Genetic Insert: hamster BiP-mCherry-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-mCherry if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62233 Copy
Species: Synthetic
Genetic Insert: VP64dCas9BFPVP64
Vector Backbone Description: Vector Backbone:TetO-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25448066
Comments: The vector backbone was from Addgene plasmid 27152 (PI Marius Wernig)
The dCas9:BFP fusion part of the insert was cloned from Addgene plasmid 44247 (PI Stanley Qi)
Proper citation: RRID:Addgene_62195 Copy
Species: Synthetic
Genetic Insert: Left CLYBL TALEN
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pZT; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25587899
Comments: pZT-C13/CLYBL TALENs were assembled using RVD monomers from Golden Gate TALEN kit 1.0 (TALEN Kit #1000000016) and a mammalian TALEN expression vector pZT
Proper citation: RRID:Addgene_62196 Copy
Species: Synthetic
Genetic Insert: A2T sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62258 Copy
Species: Synthetic
Genetic Insert: A1NT
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62255 Copy
Species: Synthetic
Genetic Insert: A1T sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62256 Copy
Species: Synthetic
Genetic Insert: su9-tagBFP
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62383 Copy
Species: Synthetic
Genetic Insert: tev 3x FLAG
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCRII blunt topo; Vector Types:yeast c-terminal tagging vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62384 Copy
Species: Synthetic
Genetic Insert: MluI-XhoI-HindIII-PstI-SalI MCS
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62397 Copy
Species: Synthetic
Genetic Insert: MluI-XhoI-PvuII-BglII-SalI MCS
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62398 Copy
Species: Synthetic
Genetic Insert: MCP-tdiRFP670
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26092696
Proper citation: RRID:Addgene_62345 Copy
Species: Synthetic
Genetic Insert: rtTA2sM2-IRES-tTSkid
Vector Backbone Description: Vector Backbone:pBlueScript II SK (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26092696
Proper citation: RRID:Addgene_62346 Copy
Species: Synthetic
Genetic Insert: mAzamiGreen
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62404 Copy
Species: Synthetic
Genetic Insert: Cerulean
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62401 Copy
Species: Synthetic
Genetic Insert: SV40 poly A
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62426 Copy
Species: Synthetic
Genetic Insert: Tamoxifen inducible Cre recombinase
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62427 Copy
Species: Synthetic
Genetic Insert: Tetracycline transactivator
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62429 Copy
Species: Synthetic
Genetic Insert: Tetracycline inducible promoter
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62422 Copy
Species: Synthetic
Genetic Insert: Bidirectional tetracycline inducible promoter
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62423 Copy
Species: Synthetic
Genetic Insert: beta-Globin poly A
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62424 Copy
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