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Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91235 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91234 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91231 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91232 Copy
Species: E. coli
Genetic Insert: AraC/PBAD
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28614382
Comments: Mariner himar1C9 transposase driven by an arabinose promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Ampicillin resistance is contained on the plasmid backbone.
For further background, see:
Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017.
Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017.
Proper citation: RRID:Addgene_91569 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~3 minutes. See author's map.
Proper citation: RRID:Addgene_8820 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~3 minutes. See author's map.
Proper citation: RRID:Addgene_8819 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~7 minutes. See author's map.
Proper citation: RRID:Addgene_8817 Copy
Species: E. coli
Genetic Insert: LacZalpha
Vector Backbone Description: Backbone Marker:Alexeyev lab; Backbone Size:3008; Vector Backbone:pMA3735; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35883613
Proper citation: RRID:Addgene_184848 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:5298; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments.
Proper citation: RRID:Addgene_186413 Copy
Species: E. coli
Genetic Insert: TrpR
Vector Backbone Description: Vector Backbone:pDestTol2pA; Vector Types:zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26838932
Proper citation: RRID:Addgene_86373 Copy
Species: E. coli
Genetic Insert: E. coli MutL
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27851738
Proper citation: RRID:Addgene_86607 Copy
Species: E. coli
Genetic Insert: E. coli MutS (D835R,R840E)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27851738
Proper citation: RRID:Addgene_86605 Copy
Species: E. coli
Genetic Insert: E. coli MutH
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27851738
Proper citation: RRID:Addgene_86609 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170413 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170414 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170411 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170412 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170405 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170403 Copy
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