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Species: Arabidopsis thaliana
Genetic Insert: UBQ10 promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
UBQ10 promoter = 2252-2884bp
BsaI site #2 = 2885-2895bp
Proper citation: RRID:Addgene_48816 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtPCS1
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pYes2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10369673
Proper citation: RRID:Addgene_49766 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, RbcS1B (AT5g38430, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50259 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, RbcS2B (AT5g38420, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50258 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, LHB1B2 (AT2g34420, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50262 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, LHB1B1 (AT2g34430, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50261 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter + 5' UTR, RbcS2B (AT5g38430, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50276 Copy
Species: Arabidopsis thaliana
Genetic Insert: CDS mature HSP21 (44-227), Arabidopsis thaliana
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM1299; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172791 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHR-GFP-VP16
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35537448
Proper citation: RRID:Addgene_183927 Copy
Species: Arabidopsis thaliana
Genetic Insert: 35S:Luc+:HSP
Vector Backbone Description: Vector Backbone:pMOD_A4800EC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176877 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUbi10
Vector Backbone Description: Vector Backbone:plCH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176871 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUbi10:Luc+:RbcsE9
Vector Backbone Description: Vector Backbone:pMOD_D4800EC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176880 Copy
Species: Arabidopsis thaliana
Genetic Insert: CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
Vector Backbone Description: Vector Backbone:pTRANS_221; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36253612
Proper citation: RRID:Addgene_176884 Copy
Species: Arabidopsis thaliana
Genetic Insert: AIDm-EGFP
Vector Backbone Description: Backbone Size:3056; Vector Backbone:pIVT; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31299080
Proper citation: RRID:Addgene_182044 Copy
Species: Arabidopsis thaliana
Genetic Insert: LbCas12a coding sequence with D156R and Arabidopsis introns
Vector Backbone Description: Backbone Size:2247; Vector Backbone:GoldenGate level 0; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.28.489853v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182384 Copy
Species: Arabidopsis thaliana
Genetic Insert: mini Singlet Oxygen Generator
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21483721
Comments: These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork.
Proper citation: RRID:Addgene_91774 Copy
Species: Arabidopsis thaliana
Genetic Insert: Auxin-inducible degron (AID)
Vector Backbone Description: Backbone Marker:Erik Jorgensen lab (Addgene #73738); Vector Backbone:pMLS280; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Comments: The auxin-inducible degron is insertered in place of a fluorescent protein in the assembled repair template. This allows tagging a gene with AID but without an FP
Proper citation: RRID:Addgene_91822 Copy
Species: Arabidopsis thaliana
Genetic Insert: Auxin-inducible degron (AID)
Vector Backbone Description: Backbone Marker:Erik Jorgensen lab (Addgene #73738); Vector Backbone:pMLS280; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91821 Copy
Species: Arabidopsis thaliana
Genetic Insert: Auxin-inducible degron (AID)
Vector Backbone Description: Backbone Marker:Erik Jorgensen lab (Addgene #73738); Vector Backbone:pMLS280; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91832 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5270; Vector Backbone:pmCherryC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28431041
Proper citation: RRID:Addgene_92035 Copy
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