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Vector Backbone Description: Backbone Size:7715; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58554 Copy
Vector Backbone Description: Backbone Size:7470; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58553 Copy
Vector Backbone Description: Backbone Size:7928; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58555 Copy
Vector Backbone Description: Backbone Size:8538; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58558 Copy
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58559 Copy
Vector Backbone Description: Backbone Size:8317; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_58551 Copy
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17038565
Comments: The V5 epitope tag serves as a linker between Dam and your favorite protein.
The lentiviral vectors can be used in two ways. When transfected as a conventional plasmid, the strong viral promoter drives high expression of the Dam(fusion) proteins. This should not be used for DamID experiments, but it is useful for checking of the proteins by Western blotting or immunofluorescence microscopy. When used as lentivirus, the strong promoter is deleted upon integration of the virus, and expression of the Dam(fusion) is now driven by the weak pIND-derived heatshock promoter.
Proper citation: RRID:Addgene_59209 Copy
Vector Backbone Description: Backbone Size:10184; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23193063
Comments: pCEP4 is from Invitrogen. CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. Gateway cassette is from Invitrogen.
Proper citation: RRID:Addgene_37626 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Zebrafish enhancer assay vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22442009
Comments: Gateway A cassette inserted between XhoI and Bglll. Please note that the cassette is not included within the depositor's sequence.
Proper citation: RRID:Addgene_37846 Copy
Vector Backbone Description: Backbone Size:15069; Vector Backbone:Lenti-CRISPR; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27458201
Proper citation: RRID:Addgene_85402 Copy
Genetic Insert: CreER
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pT3; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28009940
Proper citation: RRID:Addgene_85577 Copy
Genetic Insert: CreER
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pT3; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28009940
Proper citation: RRID:Addgene_85578 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85723 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85720 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85724 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85728 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85729 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85719 Copy
Vector Backbone Description: Backbone Size:9127; Vector Backbone:LentiGuide; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27458201
Proper citation: RRID:Addgene_85745 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86381 Copy
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