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Species: Rattus norvegicus
Genetic Insert: G-alpha-s-EE-mCerulean
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15297467
Comments: Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of αz. Effects of palmitoylation and myristoylation on αz signaling. J. Biol. Chem. 270, 9667-9675
Reference for mCerulean: Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_66084 Copy
Species: Rattus norvegicus
Genetic Insert: G-alpha-s-EE-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15297467
Comments: Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of αz. Effects of palmitoylation and myristoylation on αz signaling. J. Biol. Chem. 270, 9667-9675
Proper citation: RRID:Addgene_66083 Copy
Species: Rattus norvegicus
Genetic Insert: Opto Mu opioid receptor
Vector Backbone Description: Backbone Size:6330; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25937173
Proper citation: RRID:Addgene_66847 Copy
Species: Rattus norvegicus
Genetic Insert: ErbB2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16352662
Proper citation: RRID:Addgene_66945 Copy
Species: Rattus norvegicus
Genetic Insert: ErbB2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1D/V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17626164
Comments: A24T is a genetic variation and has no effect on function.
Proper citation: RRID:Addgene_66946 Copy
Species: Rattus norvegicus
Genetic Insert: NCX3.1
Vector Backbone Description: Vector Backbone:pBS?; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8798769
Proper citation: RRID:Addgene_67147 Copy
Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(0); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67479 Copy
Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(delta4); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67483 Copy
Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(delta2); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67481 Copy
Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(delta3); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67482 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423323
Proper citation: RRID:Addgene_114113 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin Receptor
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114289 Copy
Species: Rattus norvegicus
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:p2K7; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114311 Copy
Species: Rattus norvegicus
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114310 Copy
Species: Rattus norvegicus
Genetic Insert: IRS-3
Vector Backbone Description: Backbone Marker:pBABE-bleo/zeo is available at Addgene; Backbone Size:4888; Vector Backbone:pBABE bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: Rat IRS-3 was generated by PCR from the rat genomic DNA sequence. The intron was removed by PCR overlap extension using the following two pairs of primers: 5'-CGTGGATCCGCGATGAAGCCTGCAGGTACG-3' (sense) plus 5'-CTTGGGGGCTGAAACCCATGTTTGCTGGGCA-3' (antisense) and 5'-TGCCCAGCAAACATGGGTTTCAGCCCCCAAG-3' (sense) plus 5'-GCTGTCGACGTTCTAGAACTTGATGCTG-3' (antisense). BamHI and SalI sites were introduced at the 5' and 3' ends, respectively, of the coding sequence of IRS-3 by PCR. The subsequently generated coding sequence of rat IRS-3 was ligated into BamHI and SalI sites in pBABE-bleo.
Proper citation: RRID:Addgene_11451 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase alpha
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_33330 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: Please note that, according to NCBI numbering, the mutations are actually S99A, S494A, S510A.
Proper citation: RRID:Addgene_33320 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Proper citation: RRID:Addgene_33326 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Comments: The truncation was made by placing a stop codon at 460, but sequence was not actually removed.
The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_33324 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21807092
Proper citation: RRID:Addgene_33329 Copy
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