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Species: Mus musculus
Genetic Insert: 3xHA-SAPAP3
Vector Backbone Description: Backbone Size:5086; Vector Backbone:PZac2.1; Vector Types:Bacterial Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37046092
Proper citation: RRID:Addgene_190200 Copy
Species: Homo sapiens
Genetic Insert: Codon optimized VPS13D
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:6000; Vector Backbone:pcDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33891013
Proper citation: RRID:Addgene_194006 Copy
Species: Bacteria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:2382; Vector Backbone:pZ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30867677
Proper citation: RRID:Addgene_193791 Copy
Species: Homo sapiens
Genetic Insert: U7 snRNA antisense CypA Exon 4
Vector Backbone Description: Backbone Size:6114; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28624228
Comments: Please note that the Addgene verified sequence differs from the depositor's reference sequence. These minor differences should not affect plasmid function.
Proper citation: RRID:Addgene_190695 Copy
Species: Homo sapiens
Genetic Insert: U7 snRNA
Vector Backbone Description: Backbone Size:6114; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28624228
Comments: Please note that the Addgene verified sequence differs from the depositor's reference sequence. These minor differences should not affect plasmid function.
Proper citation: RRID:Addgene_190694 Copy
Species: N. meningitidis
Genetic Insert: NmeABE8e
Vector Backbone Description: Backbone Size:3486; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35811581
Proper citation: RRID:Addgene_199263 Copy
Species: N. meningitidis
Genetic Insert: NmeABE8e
Vector Backbone Description: Backbone Size:3348; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35811581
Proper citation: RRID:Addgene_199262 Copy
Species: Mutated Lambda CI
Genetic Insert: Frame-shifted CI
Vector Backbone Description: Backbone Size:2009; Vector Backbone:pZ; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30867677
Proper citation: RRID:Addgene_193778 Copy
Species: Synthetic
Genetic Insert: proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
Vector Backbone Description: Vector Backbone:JAB2155; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35951698
Comments: Backbone JAB2155 contains proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190386 Copy
Species: Homo sapiens
Genetic Insert: RXRG
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_001256571,NM_001256570,ENST00000619224 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_142027 Copy
Species: Bacteriophage lambda
Genetic Insert: Lambda CI
Vector Backbone Description: Backbone Size:2009; Vector Backbone:pZ; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35024106
Proper citation: RRID:Addgene_193773 Copy
Species: Bacteriophage lambda
Genetic Insert: Lambda CI
Vector Backbone Description: Backbone Size:2009; Vector Backbone:pZ; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35024106
Proper citation: RRID:Addgene_193775 Copy
Genetic Insert: Chloramphenicol resistance gene - 111TAG
Vector Backbone Description: Vector Backbone:pREP; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36383641
Proper citation: RRID:Addgene_197571 Copy
Species: Salmonella enterica serovar Typhimurium
Genetic Insert: PsopD2 sopD2 deltaCC::L16::HaloTag::HA
Vector Backbone Description: Vector Backbone:pWSK29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_198157 Copy
Species: Bacteria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:2234; Vector Backbone:pZ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35024106
Proper citation: RRID:Addgene_193756 Copy
Species: Homo sapiens
Genetic Insert: CDC42BPB-V5-miniTurbo
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_198008 Copy
Species: Homo sapiens
Genetic Insert: ALPK2-V5-miniTurbo
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_198009 Copy
Species: Homo sapiens
Genetic Insert: miniTurbo-V5-NEK11
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_198005 Copy
Genetic Insert: YP_001426521
Vector Backbone Description: Vector Backbone:pBZS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36333700
Proper citation: RRID:Addgene_198363 Copy
Genetic Insert: YP_001497284
Vector Backbone Description: Vector Backbone:pBZS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36333700
Proper citation: RRID:Addgene_198364 Copy
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