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Species: Saccharomyces cerevisiae
Genetic Insert: SUP35 NM-domain
Vector Backbone Description: Backbone Size:5276; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11606530
Proper citation: RRID:Addgene_1255 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLPo
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37290446
Comments: Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_125576 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mutant HSP104
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC25S; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158570
Proper citation: RRID:Addgene_1300 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc20/mCherry-AID(71-114)-tCYC1-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31606371
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc20 in yeast.
The work is currently under review.
Proper citation: RRID:Addgene_130270 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y257W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L892W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1308 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y257W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1310 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y466W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1311 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y507W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1312 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc3 + Cdc10-SNAP
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression, bicistronic; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23889817
Comments: combine with pET-Cdc12-Cdc11 for expression of SNAP-tagged septin rods
Proper citation: RRID:Addgene_131161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y819W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1314 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (361-548)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4746; Vector Backbone:pPROEX-HTb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1317 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 20mer GFP cassette guide (gGFP) and 5' sgRNA
Vector Backbone Description: Backbone Size:12364; Vector Backbone:pML107 (addgene #67639); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31738765
Proper citation: RRID:Addgene_131784 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 20mer CASS5a guide (gCASS5a) and 5' sgRNA
Vector Backbone Description: Backbone Size:12364; Vector Backbone:pML107 (addgene #67639); Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31738765
Proper citation: RRID:Addgene_131774 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (1-575)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4746; Vector Backbone:pPROEX-HTb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1318 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YPD1 protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5493; Vector Backbone:pET22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10543964
Proper citation: RRID:Addgene_12655 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ALD6
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27419466
Proper citation: RRID:Addgene_126751 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: adh2, acs1*, ald6
Vector Backbone Description: Backbone Marker:Liu Yiqi; Backbone Size:6469; Vector Backbone:pPIC 3.5K(DHis4); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Comments: Depositor confirms the following mutations A30S and S629N do not affect function.
Proper citation: RRID:Addgene_126739 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: adh2
Vector Backbone Description: Backbone Marker:Liu Yiqi; Backbone Size:6469; Vector Backbone:pPIC 3.5K(DHis4); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126734 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: acs1*
Vector Backbone Description: Backbone Marker:Liu Yiqi; Backbone Size:6469; Vector Backbone:pPIC 3.5K(DHis4); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Comments: Dep. confirms the following mutations A30S, P113S, and S629N do not affect function.
Proper citation: RRID:Addgene_126736 Copy
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