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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
His8:MBP-tev-Phe-eΔK-Flv
 
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RRID:Addgene_83395 flavin binding protein MioC E. coli Ampicillin Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-09-01 10:03:30 0
pACYCDuet_F31YG121VDHFR_R166QTS
 
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RRID:Addgene_91228 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol F31YG121 2026-09-01 10:05:11 0
pACYCDuet_F31VDHFR_R166QTS
 
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RRID:Addgene_91245 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol F31V 2026-09-01 10:05:12 0
pACYCDuet_W22HDHFR_WTTS
 
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RRID:Addgene_91241 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol W22H 2026-09-01 10:05:12 0
pACYCDuet_M42FG121VDHFR_WTTS
 
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RRID:Addgene_91239 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol M42FG121V 2026-09-01 10:05:12 0
pACYCDuet_M42FDHFR_WTTS
 
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RRID:Addgene_91237 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol M42F 2026-09-01 10:05:12 0
pACYCDuet_L54IDHFR_WTTS
 
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RRID:Addgene_91235 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol L54I 2026-09-01 10:05:11 0
pACYCDuet_L54IDHFR_R166QTS
 
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RRID:Addgene_91234 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol L54I 2026-09-01 10:05:11 0
pACYCDuet_F31YL54IDHFR_WTTS
 
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RRID:Addgene_91231 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol F31YL54I 2026-09-01 10:05:11 0
pACYCDuet_G121VDHFR_R166QTS
 
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RRID:Addgene_91232 folA E. coli Chloramphenicol PMID:31189117 Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol G121V 2026-09-01 10:05:11 0
pSAM_AraC
 
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1+ mentions
RRID:Addgene_91569 AraC/PBAD E. coli Ampicillin and Kanamycin PMID:28614382 Mariner himar1C9 transposase driven by an arabinose promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Ampicillin resistance is contained on the plasmid backbone. For further background, see: Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017. Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017. Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin 2026-09-01 10:05:17 5
pAT-9218
 
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RRID:Addgene_124225 SpCas9 E. coli Chloramphenicol PMID:33450024 Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-09-01 09:15:49 0
psgRNA Library
 
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RRID:Addgene_115927 E. coli gRNAs E. coli Kanamycin PMID:29765036 Vector Backbone:psgRNAcos; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-09-01 09:13:29 0
pJB332
 
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RRID:Addgene_115949 Lvl0 TetR-Gly9-DDX6 (contains SapI) E. coli Ampicillin PMID:30327560 Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin 2026-09-01 09:13:29 0
pLXI_TRC401 LacZ
 
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1+ mentions
RRID:Addgene_111183 lacZ E. coli Ampicillin PMID:30860482 Backbone Marker:Broad Institute; Backbone Size:9354; Vector Backbone:pLXI_TRC403; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 09:12:08 1
pLXI_TRC403 LacZ
 
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1+ mentions
RRID:Addgene_111184 lacZ E. coli Ampicillin PMID:30860482 Backbone Marker:Broad Institute; Backbone Size:9396; Vector Backbone:pLXI_TRC403; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 09:12:08 2
pPagP
 
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RRID:Addgene_112125 PagP E. coli Ampicillin PMID:18641391 Backbone Size:5600; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal signal sequence not included and replaced with Methionine 2026-09-01 09:12:24 0
pRK2-AraE
 
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1+ mentions
RRID:Addgene_110141 araE E. coli Gentamicin PMID:29299733 Vector Backbone:pSEVA221; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin 2026-09-01 09:11:52 1
pCK314
 
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RRID:Addgene_110545 Modified PrhaBAD (CRP-binding sites removed) E. coli Kanamycin PMID:29544054 Backbone Size:3941; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:11:58 0
pCK306
 
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RRID:Addgene_110544 PrhaBAD E. coli Kanamycin PMID:29544054 Backbone Size:3941; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:11:58 0

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