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Species: Saccharomyces cerevisiae
Genetic Insert: GCN4-based single uORF-containing 5'-UTR
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38220 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' 1770 bp from STU2 ORF
Vector Backbone Description: Backbone Marker:GE healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22993214
Proper citation: RRID:Addgene_38314 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' 918 bp from STU2 ORF
Vector Backbone Description: Backbone Marker:GE healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22993214
Proper citation: RRID:Addgene_38315 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PPX1
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16184370
Proper citation: RRID:Addgene_38327 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUS2p
Vector Backbone Description: Backbone Marker:Midwest Center for Structural Genomics; Backbone Size:5704; Vector Backbone:p11; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26631165
Proper citation: RRID:Addgene_73060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Proper citation: RRID:Addgene_73285 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Proper citation: RRID:Addgene_73289 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Proper citation: RRID:Addgene_73286 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Comments: Please note that mutation A40T in nourseothricin was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_73291 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Comments: Please note that mutation A40T in nourseothricin was foud during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_73295 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Venus
Vector Backbone Description: Backbone Size:10700; Vector Backbone:pNIA-CEN; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089030
Proper citation: RRID:Addgene_73618 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LINXa4-Bre1dNLS
Vector Backbone Description: Backbone Size:8084; Vector Backbone:pNIA-CEN; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089030
Proper citation: RRID:Addgene_73617 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2 I149A
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_73887 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Erp1 CT
Vector Backbone Description: Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_73920 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2 I155A
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_73888 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Inducible peptide of the dPSTR
Vector Backbone Description: Backbone Size:5053; Vector Backbone:pRS305; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This intermediate cloning vector is used to change the measured promoter (here pSTL11), by cloning it between SacI (or AatII) and XbaI (or SpeI). The entire inducible peptide has to be inserted into a pSIV by cloning AatII / SphI (into pDA183 or pDA200 for instance).
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74249 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1-dPSTRr(2,1)
Vector Backbone Description: Vector Backbone:pSIVU; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This vector contains the complete pSTL1-dPSTRr(2,1). The vector can be cut either PacI or BstBI prior to transformation in yeast. Selection in Ura depleted medium.
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74247 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: single chain Cse4-Scm3-H4 with 2 Thrombin site
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pET42+b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21412236
Proper citation: RRID:Addgene_28250 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: single chain Cse4-H4 with Thrombin site
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pET42+b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21412236
Proper citation: RRID:Addgene_28251 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP45 gene encoding translation release factor 1 from S. cerevisiae
Vector Backbone Description: Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20444877
Proper citation: RRID:Addgene_29383 Copy
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