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Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83395 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91228 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91245 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91241 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91239 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91237 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91235 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91234 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91231 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91232 Copy
Species: E. coli
Genetic Insert: AraC/PBAD
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28614382
Comments: Mariner himar1C9 transposase driven by an arabinose promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Ampicillin resistance is contained on the plasmid backbone.
For further background, see:
Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017.
Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017.
Proper citation: RRID:Addgene_91569 Copy
Species: E. coli
Genetic Insert: SpCas9
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33450024
Proper citation: RRID:Addgene_124225 Copy
Species: E. coli
Genetic Insert: E. coli gRNAs
Vector Backbone Description: Vector Backbone:psgRNAcos; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_115927 Copy
Species: E. coli
Genetic Insert: Lvl0 TetR-Gly9-DDX6 (contains SapI)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115949 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:9354; Vector Backbone:pLXI_TRC403; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30860482
Proper citation: RRID:Addgene_111183 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:9396; Vector Backbone:pLXI_TRC403; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30860482
Proper citation: RRID:Addgene_111184 Copy
Species: E. coli
Genetic Insert: PagP
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18641391
Proper citation: RRID:Addgene_112125 Copy
Species: E. coli
Genetic Insert: araE
Vector Backbone Description: Vector Backbone:pSEVA221; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29299733
Proper citation: RRID:Addgene_110141 Copy
Species: E. coli
Genetic Insert: Modified PrhaBAD (CRP-binding sites removed)
Vector Backbone Description: Backbone Size:3941; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29544054
Proper citation: RRID:Addgene_110545 Copy
Species: E. coli
Genetic Insert: PrhaBAD
Vector Backbone Description: Backbone Size:3941; Vector Backbone:pACYC177; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29544054
Proper citation: RRID:Addgene_110544 Copy
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