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Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139896 Copy
Species: E. coli
Genetic Insert: MotA
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28974721
Proper citation: RRID:Addgene_158437 Copy
Species: E. coli
Genetic Insert: MotA
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28974721
Proper citation: RRID:Addgene_158436 Copy
Species: E. coli
Genetic Insert: MotA
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28974721
Proper citation: RRID:Addgene_158434 Copy
Species: E. coli
Genetic Insert: ccdB
Vector Backbone Description: Backbone Size:6064; Vector Backbone:ptac; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16705187
Proper citation: RRID:Addgene_15922 Copy
Species: E. coli
Genetic Insert: RNase III
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36493370
Proper citation: RRID:Addgene_189537 Copy
Species: E. coli
Genetic Insert: MG1655-C321 mutS+, λ-, Δ(ybhB-bioAB)::zeoR, ΔprfA, ΔserB
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36280721
Comments: MG1655-C321 -- All 321 UAG codons changed to UAA.
mutS+ -- Restores normal spontaneous mutation frequency.
(λ-) – Deleted Lambda Phage operon to allow growth at 37C.
Δ(ybhB-bioAB)::zeoR – Deletion of biotin biosynthetic operon using Zeocin cassette; strain is d-biotin auxotroph.
ΔprfA – Deletion of polypeptide release factor 1 (RF1) to prevent translation termination at UAG codons.
ΔserB – Deletion of phosphoserine phosphatase to provide intracellular source of phosphoserine; strain is Serine auxotroph.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1)
Proper citation: RRID:Addgene_192872 Copy
Species: E. coli
Genetic Insert: Encodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase CitA: Tar[1-43]-CitA[55-173]-Tar[184-553]
Vector Backbone Description: Backbone Size:4211; Vector Backbone:pKG116; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27285081
Proper citation: RRID:Addgene_82029 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83389 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83388 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83383 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83384 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83387 Copy
Species: E. coli
Genetic Insert: Mechanosensitive channel of large conductance
Vector Backbone Description: Vector Backbone:pADtet; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25606062
Proper citation: RRID:Addgene_83373 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83390 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28277608
Proper citation: RRID:Addgene_83391 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83396 Copy
Species: E. coli
Genetic Insert: flavin binding protein MioC
Vector Backbone Description: Vector Backbone:pVP16; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_83395 Copy
Species: E. Coli
Genetic Insert: tet operator
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10440378
Comments: the tet operator can be released from this plasmid using a XhoI digest.
The construct is composed of the tet operator sequences followed by a CMV minimal promoter. The insert was digested with XhoI and EcoRI, klenow filled, and cloned into the SmaI site of pBluescript KS. See author's map for more information.
Proper citation: RRID:Addgene_8901 Copy
Species: E. coli
Genetic Insert: yhjH
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3719; Vector Backbone:pCOLADuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27382070
Proper citation: RRID:Addgene_79165 Copy
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