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Species: Saccharomyces cerevisiae
Genetic Insert: MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
Vector Backbone Description: Backbone Size:6000; Vector Backbone:plIVMD156; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Proper citation: RRID:Addgene_160935 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
Vector Backbone Description: Backbone Size:6000; Vector Backbone:plIVMD156; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Comments: Please note that the T247A mutation in sfGFP does not impact plasmid function.
Proper citation: RRID:Addgene_160936 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-mTagBFP2-HA Tag
Vector Backbone Description: Vector Backbone:psh100; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Proper citation: RRID:Addgene_160939 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHF1
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin
Comments: This is an improved version of the plasmid reported here:
https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X
https://doi.org/10.1016/j.str.2019.12.002
All histone genes code for S. cerevisiae proteins. Expression and purification procedures are as described.
Proper citation: RRID:Addgene_160930 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHF1
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin
Comments: This is an improved version of the plasmid reported here:
https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X
https://doi.org/10.1016/j.str.2019.12.002
All histone genes code for S. cerevisiae proteins.
Proper citation: RRID:Addgene_160929 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pol1
Vector Backbone Description: Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32923642
Proper citation: RRID:Addgene_160990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pol1
Vector Backbone Description: Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32923642
Proper citation: RRID:Addgene_160988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast genes
Vector Backbone Description: Backbone Marker:Addgene 35452; Vector Backbone:pRS41x; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33757429
Proper citation: RRID:Addgene_161769 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: COF1-RFP
Vector Backbone Description: Vector Backbone:pRS413-HIS,Amp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20332110
Proper citation: RRID:Addgene_37101 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF4E
Vector Backbone Description: Backbone Marker:EMD bioscience; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37228 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF4E
Vector Backbone Description: Backbone Marker:EMD bioscience; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37229 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF5B
Vector Backbone Description: Vector Backbone:pSKB3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17913637
Proper citation: RRID:Addgene_37221 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHtRNA
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17913637
Comments: HHtRNA sequence (rev orientation): TGGTAGCGCCGCTCGGTTTCGATCCGAGGACATCAGGGTTATGAGCCCTGCGCGCTTCCACTGCGCCACGGCGCTGACGGTACCGGGTACCGTTTCGTCCTCACGGACTCATCAGAGCG
Proper citation: RRID:Addgene_37222 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17913637
Proper citation: RRID:Addgene_37217 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF5
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17913637
Proper citation: RRID:Addgene_37219 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HRC1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20864040
Proper citation: RRID:Addgene_37233 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Met3p-mCherry-Tub1
Vector Backbone Description: Backbone Size:4381; Vector Backbone:pRS306; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21294277
Proper citation: RRID:Addgene_37554 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ras2 C-term (last 9 aa)
Vector Backbone Description: Backbone Size:4749; Vector Backbone:pKT0128; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19755860
Proper citation: RRID:Addgene_37557 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tandem PA-GFP
Vector Backbone Description: Backbone Size:4882; Vector Backbone:pDH3; Vector Types:Yeast tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18727145
Proper citation: RRID:Addgene_37548 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38228 Copy
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