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Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, donor vector (human); Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26707206
Comments: Deleted T2 protospacer sequence in HA-R
Proper citation: RRID:Addgene_80490 Copy
Vector Backbone Description: Backbone Size:5669; Vector Backbone:pBIG2abcd; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27114506
Proper citation: RRID:Addgene_80618 Copy
Vector Backbone Description: Vector Backbone:Binary vector pAMPAT-MCS; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_80681 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:10726; Vector Backbone:pBPGw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-RR-promoter is a Gateway compatible vector enabling high throughput cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Renilla-luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Renilla-luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83343 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:11536; Vector Backbone:pBPGUw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-GL-enhancer is a Gateway compatible vector enabling high throughput cloning of enhancer elements upstream of the Drosophila synthetic core promoter (DSCP) and a GFP::Luciferase(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase, and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83338 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:10876; Vector Backbone:pBPGUw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-RR-enhancer is a Gateway compatible vector enabling high throughput cloning of enhancer elements upstream of the Drosophila synthetic core promoter (DSCP) and a mCherry::Renilla(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Renilla-luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Renilla-luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83339 Copy
Species: Phaeodactylum tricornutum
Genetic Insert: TALEN Backbone
Vector Backbone Description: Backbone Marker:GenBank_NCBI: JN180663; Backbone Size:3860; Vector Backbone:pPha-NR; Vector Types:Phaeodactylum tricornutum; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: N.E. Sanjana, L. Cong, Y. Zhou, M.M. Cunniff, G. Feng, F. Zhang
A transcription activator-like effector toolbox for genome engineering
Nat. Protoc., 7 (2012), pp. 171–192
Proper citation: RRID:Addgene_90421 Copy
Species: Phaeodactylum tricornutum
Genetic Insert: TALEN Backbone
Vector Backbone Description: Backbone Marker:GenBank_NCBI: JN180663; Backbone Size:3860; Vector Backbone:pPha-NR; Vector Types:Phaeodactylum tricornutum; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: N.E. Sanjana, L. Cong, Y. Zhou, M.M. Cunniff, G. Feng, F. Zhang
A transcription activator-like effector toolbox for genome engineering
Nat. Protoc., 7 (2012), pp. 171–192
Proper citation: RRID:Addgene_90423 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco-aphA
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81013 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco-PMKco
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81016 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81017 Copy
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLV-CMV-y/hNubI-tripleFLAG-linker-Gateway-PuroR; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_122848 Copy
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:7720; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123216 Copy
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:7720; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123215 Copy
Vector Backbone Description: Backbone Size:12314; Vector Backbone:pPW; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27006187
Proper citation: RRID:Addgene_124238 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9367; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11517 Copy
Species: Synthetic
Genetic Insert: Lvl0 3'UTR Dest
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115952 Copy
Vector Backbone Description: Vector Backbone:pDEST-Tol2-pA2; Vector Types:Zebrafish plasmids; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_109731 Copy
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6000; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16322578
Comments: The MBP ORF can be sequenced using 5'-GAG CGG ATA ACA ATT TCA CAC AGG-3' as a tac promoter sequencing primer (in the forward direction). To sequence inserts fused to the C-terminus of MBP, the primers are 5'-GAT GAA GCC CTG AAA GAC GCG CAG-3' (forward) and 5'-GCA AGG CGA TTA AGT TGG GTA ACG C-3' (reverse).
Proper citation: RRID:Addgene_11085 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmec-4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5020; Vector Backbone:Gateway Destination Vector; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20203177
Proper citation: RRID:Addgene_110883 Copy
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