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Species: Arabidopsis thaliana
Genetic Insert: RabG3B
Vector Backbone Description: Vector Backbone:pUBN-RFP; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_102371 Copy
Species: Arabidopsis thaliana
Genetic Insert: RabG3B
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102372 Copy
Species: Arabidopsis thaliana
Genetic Insert: EFR
Vector Backbone Description: Vector Backbone:pEarleyGate103; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_102413 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B (1-621 aa)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB621-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB621-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100282 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome interacting factor 3
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PIF3-mEGFP in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
Proper citation: RRID:Addgene_100283 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Proper citation: RRID:Addgene_100538 Copy
Species: Arabidopsis thaliana
Genetic Insert: OEP7(1–50)
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28634853
Proper citation: RRID:Addgene_100601 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B (1-908 aa, Y276H mutation)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB-Y276H-mCherry-HRasCT in mammalian cells.
PhyB-Y276H has two additional missense mutations (P840S, P884L); the construct will function as described in the publication.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100540 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-LS-F138I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100922 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-GS-L255I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100966 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-GS
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100967 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTryoshka1;3-GS-F138I
Vector Backbone Description: Backbone Size:6939; Vector Backbone:pDRf1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28874729
Proper citation: RRID:Addgene_100965 Copy
Species: Arabidopsis thaliana
Genetic Insert: ZF108_3xFLAG_YPet
Vector Backbone Description: Vector Backbone:pMDC123; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29444862
Proper citation: RRID:Addgene_106441 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK32
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Proper citation: RRID:Addgene_107432 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK32
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Comments: Addgene NGS results confirmed that this plasmid contains amino acids 1-326 of the CPK32 translation
Proper citation: RRID:Addgene_107431 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK30
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Proper citation: RRID:Addgene_107430 Copy
Species: Arabidopsis thaliana
Genetic Insert: CPK10
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28489820
Comments: Addgene NGS results confirmed that this plasmid contains amino acids 1-326 of the CPK10 translation
Proper citation: RRID:Addgene_107427 Copy
Species: Arabidopsis thaliana
Genetic Insert: Ath-HEN1
Vector Backbone Description: Backbone Size:7517; Vector Backbone:pCFJ350-ttTi5605; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29481550
Proper citation: RRID:Addgene_108362 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1
Vector Backbone Description: Vector Backbone:pK7GWIWG2; Vector Types:Plant Expression, RNAi; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Comments: Full sequence is not available for this plasmid. Please see the diagnostic digest and partial sequencing results as references for verification
To build the pRCH1:HDT1HDT2 RNAi construct (hdt1,2i), the CaMV 35S promoter in pK7GWIWG2 vector (Limpens et al., 2005) was replaced with RCH1 promoter [pK7GWIWG2(II)]. The RCH1 promoter was first amplified on genomic DNA and cloned into pENTR-D-TOPO. Then it was cut out from the vector by HindIII (partial digestion) and XbaI and recombined with two fragments of the pK7GWIWG2(II) vector in a three-point ligation. The two fragments of pK7GWIWG2(II) vector were obtained by digestion either with HindII and NcoI, or with SpeI (compatible with XbaI) and HindIII. The whole RNAi cassette including the RCH1 promoter was cut out from the vector using ApaI and HindIII and ligated into the pBnRGW binary vector digested with the same enzymes and in such way creating pBnRRGWIWG vector. The RNAi target sequences of HDT1 and HDT2 coding sequences (0.6 kb for each) were combined in one amplicon using a two-step PCR, following the procedure described by Franssen et al. (2015). In brief, the first step PCRs introduced short overlaps (15 bp) in PCR fragments of HDT1 and HDT2 coding sequences with HDT1rnai-F and HDT1rnai-R primers, or with HDT2rnai-F and HDT2rnai-R primers. These two fragments were used as templates in the second-step PCR with HDT1rnai-F and HDT2rnai-R primers. The final PCR fragment was introduced into pENTR-D-TOPO vector and recombined in inverse-repeat orientation into the pBnRRGWIWG binary vector by a LR Gateway reaction (Invitrogen).
Proper citation: RRID:Addgene_108446 Copy
Species: Arabidopsis thaliana
Genetic Insert: HDT1
Vector Backbone Description: Vector Backbone:pBnRGW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28855334
Proper citation: RRID:Addgene_108565 Copy
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