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Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Size:3462; Vector Backbone:pQE30 (QIAGEN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:12761287
Proper citation: RRID:Addgene_16232 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDC3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11577116
Proper citation: RRID:Addgene_16252 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34469745
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.05.425451v1 for bioRxiv preprint.
Please note: Plasmid contains E10G and N257D mutations in URA3. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_174064 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGal1-LacO-3NLS-CIB1-VP16-tADH1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34469745
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.05.425451v1 for bioRxiv preprint.
Please note: The plasmid contains a 217bp insertion between Tadh1 and HIS3 3'UTR compared to the depositor's provided sequence. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_174066 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEF1-3NLS-97-3ZF-VP16
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34469745
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.05.425451v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_174068 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34469745
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.01.05.425451v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_174063 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GALL promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Size:2574; Vector Backbone:p404ADH1; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_17418 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET3 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Size:2581; Vector Backbone:p404ADH1; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_17419 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:2691; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_17437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 11R Unique
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:3956; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34702734
Proper citation: RRID:Addgene_174830 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Met4 UIML
Vector Backbone Description: Vector Backbone:pet28(a); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34763062
Proper citation: RRID:Addgene_174888 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: S. cerevisiae TEF2 promoter
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pFA6a–link–yEGFP–Kan; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33311538
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.11.089300v1 for bioRxiv preprint.
Parent vector is described in "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae", Mark A Sheff , Kurt S ThornYeast 2004 Jun;21(8):661-70. doi: 10.1002/yea.1130. The parent vector was intended for GFP tagging of yeast proteins. We placed the TEF2 promoter directly upstream of GFP for sugar-insensitive expression of GFP under galactose or dextrose conditions.
Proper citation: RRID:Addgene_160432 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP2
Vector Backbone Description: Backbone Marker:self-made; derived from the BioBrick assembly plasmid pSB1C3 ; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160599 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNA Polymerase II CTD
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468530
Proper citation: RRID:Addgene_160688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CIM5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16810255
Proper citation: RRID:Addgene_16074 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ull1 delta440
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12761287
Proper citation: RRID:Addgene_16087 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11577116
Proper citation: RRID:Addgene_16092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBC9
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12761287
Proper citation: RRID:Addgene_16090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VRG4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Comments: Parental strain JK93da.
Proper citation: RRID:Addgene_160904 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Medium-chain fatty acid ethyl ester synthase
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32886882
Proper citation: RRID:Addgene_160864 Copy
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