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Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 3C-like proteinase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725301.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149209 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149210 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724394.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149211 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725302.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149212 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725302.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149215 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725255.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149219 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 M (membrane)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724393.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149220 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149223 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mutant HSP104
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC25S; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158570
Proper citation: RRID:Addgene_1300 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc20/mCherry-AID(71-114)-tCYC1-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31606371
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc20 in yeast.
The work is currently under review.
Proper citation: RRID:Addgene_130270 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y257W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L892W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1308 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y257W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1310 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y466W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1311 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y507W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1312 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc3 + Cdc10-SNAP
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression, bicistronic; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23889817
Comments: combine with pET-Cdc12-Cdc11 for expression of SNAP-tagged septin rods
Proper citation: RRID:Addgene_131161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OsTIR1(WT)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140655 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL-OsTIR1(F74G)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140656 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL-OsTIR1(F74A)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140657 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: COX4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4664; Vector Backbone:pAcGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32328629
Proper citation: RRID:Addgene_141153 Copy
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