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Vector Backbone Description: Vector Backbone:Other; Vector Types:subcloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_231172 Copy
Genetic Insert: FRT10-STOP-FRT10
Vector Backbone Description: Vector Backbone:pBPGAL4DBD; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39677673
Comments: Please visit https://doi.org/10.1101/2024.12.02.626507 for bioRxiv preprint.
Proper citation: RRID:Addgene_232831 Copy
Vector Backbone Description: Vector Backbone:pCAGEN_DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229834 Copy
Vector Backbone Description: Vector Backbone:pB-pRAM-Flex-mClover3-KrasG12D-WPRE-pA-EFS-TetR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229828 Copy
Species: Danio rerio
Genetic Insert: 4xU6:sgRNA
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_227778 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36433 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36434 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36435 Copy
Genetic Insert: Gateway Cassette A R2-R1
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36428 Copy
Vector Backbone Description: Backbone Marker:Voytas lab (Addgene Plasmid #35397); Backbone Size:13853; Vector Backbone:pCP5; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21493687
Comments: pCP5b is a high copy derivative of pCP5 for higher DNA yield from bacteria.
Plasmid used to assay TALEN function in yeast. Clone target sequences into BglII and SpeI sites as described on article.
Grow yeast transformants in SD-Ura-Trp prior to assay to prevent spontaneous recombination between the lacZ fragments
Proper citation: RRID:Addgene_37185 Copy
Vector Backbone Description: Vector Backbone:PB-TAG-ERN; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33080218
Proper citation: RRID:Addgene_164521 Copy
Species: SARS-CoV-2
Genetic Insert: nsp12-3xFlag/nsp7-His6-nsp8
Vector Backbone Description: Vector Backbone:pBIG2abc (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169183 Copy
Vector Backbone Description: Vector Backbone:pmCellFree_KA3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_169408 Copy
Vector Backbone Description: Vector Backbone:pCG150 - Addgene plasmid 17247; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34791215
Comments: Please visit https://doi.org/10.1101/2020.11.25.398784 for bioRxiv preprint.
Proper citation: RRID:Addgene_169693 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:8902; Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18621688
Comments: pBPGw is a modular Gateway compatible promoter-less GAL4 vector amenable to high throughput in vitro cloning using Invitrogen LR clonase and specific in vivo genomic targeting using PhiC31 integrase. In addition the GAL4 CDS and yeast transcriptional terminator can easily be substituted for another driver by a directional 5' KpnI to 3' HindIII digest.
Proper citation: RRID:Addgene_17574 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9052; Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18621688
Comments: pBPGUw is a modular Gateway compatible GAL4 vector amenable to high throughput in vitro cloning using Invitrogen LR clonase and specific in vivo genomic targeting using PhiC31 integrase. pBPGUw contains a Drosophila synthetic core promoter (DSCP) that contains TATA, Inr, MTE, and DPE motifs. In addition the GAL4 CDS and yeast transcriptional terminator can easily be substituted for another driver by a directional 5' KpnI to 3' HindIII digest.
Proper citation: RRID:Addgene_17575 Copy
Genetic Insert: Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
Vector Backbone Description: Vector Backbone:pQE-30; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35920321
Proper citation: RRID:Addgene_176225 Copy
Genetic Insert: Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
Vector Backbone Description: Vector Backbone:pQE-30; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35920321
Proper citation: RRID:Addgene_176226 Copy
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42153 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42154 Copy
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