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Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33225 Copy
Species: Caenorhabditis elegans
Genetic Insert: aex-4/tag-81 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33185 Copy
Species: Caenorhabditis elegans
Genetic Insert: aex-4/tag-81 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33186 Copy
Species: Caenorhabditis elegans
Genetic Insert: T23E1.1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33183 Copy
Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33214 Copy
Species: Caenorhabditis elegans
Genetic Insert: nsy-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33171 Copy
Species: Caenorhabditis elegans
Genetic Insert: fkh-8 3' UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33174 Copy
Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33218 Copy
Species: Caenorhabditis elegans
Genetic Insert: ptp-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33169 Copy
Species: Caenorhabditis elegans
Genetic Insert: hlh-8 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33163 Copy
Species: Caenorhabditis elegans
Genetic Insert: F55G1.12 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33164 Copy
Species: Caenorhabditis elegans
Genetic Insert: beta-tubulin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Comments: 1st generation pie-1 vector
Proper citation: RRID:Addgene_26951 Copy
Species: Caenorhabditis elegans
Genetic Insert: lsm-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26939 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRA-2 GFP
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pPD95.79; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20921392
Proper citation: RRID:Addgene_27128 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRA-2 FLAG
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pPD95.79; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20921392
Comments: Cloning of this plasmid was achieved in the following steps:
(1)the sequence 3' (3'UTR) of the tags was added by (EcoRI/SpeI)
(2) 1.2 kb XhoI/KpnI tra-2 pcr fragment into SalI/KpnI cut vector (destroying one SalI/XhoI site but adding another in the insert) immediately 5' of the tag
(3) 7 kb HindIII/SalI fragment into same that partially replaced fragment added in step 2 containing most of the TRA-2 coding sequence
(4) 5 kb Xho/AgeI that added the promoter and upstream gene in the operon as well as some Tra-2 coding sequence
Proper citation: RRID:Addgene_27129 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmyo-3::cfp::lacZ::unc-54_3'UTR
Vector Backbone Description: Backbone Size:2000; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Comments: Please note that there are some slight discrepancies between Addgene's quality control sequence and the theoretical sequence from the Fire lab. These differences should not affect function.
Proper citation: RRID:Addgene_27510 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:TOPO; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20493184
Proper citation: RRID:Addgene_27504 Copy
Species: Caenorhabditis elegans
Genetic Insert: phsp16.41::GFP-F64LS65T::(Gly)5Ala
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21544 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119:mCherry
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:300; Vector Backbone:R6K origin PCR fragment; Vector Types:Worm Expression, Adds unc-119 marker to Amp resistant plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19520111
Comments: This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
The other mismatches in 21596 are in a polylinker between the KanR gene and the unc-119 3’ UTR and should not cause any problems.
Proper citation: RRID:Addgene_21596 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5 (w/o ATG)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2643; Vector Backbone:pDONR P4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21512 Copy
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