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Species: Saccharomyces cerevisiae
Genetic Insert: gRNA_RNH1_RNH201
Vector Backbone Description: Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177271 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pRS415U; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177272 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t:T7p:cgRNA_HIS3_stop:tZ
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pEDJ400; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Proper citation: RRID:Addgene_177275 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1t:HIS3_23∆29-XII-5 :<-PGK1p
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCfB2909; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107026
Comments: Integrative plasmid
Please note: Plasmid contains a 68bp insertion in mADH1t compared to the depositor's provided sequence. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_177274 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSpeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10197526
Proper citation: RRID:Addgene_17748 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mCherry
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177706 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
Vector Backbone Description: Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35069506
Proper citation: RRID:Addgene_177711 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: S000001911
Vector Backbone Description: Vector Backbone:pROS13; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36849832
Comments: Cloning details are in the Methods section and Supplementary Table 2 of the paper.
Proper citation: RRID:Addgene_196613 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pab1
Vector Backbone Description: Backbone Size:7167; Vector Backbone:pOBD2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25671604
Comments: pOBD2 + NcoI-PAB1(369-612 bp)-SalI. Expresses Pab1 RRM2 fragment with E181R mutation. Expresses Pab1 fragment fused to GAL4BD.
Proper citation: RRID:Addgene_196835 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eIF4G1
Vector Backbone Description: Backbone Size:8429; Vector Backbone:pOAD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25671604
Comments: pOAD + EcoRI-eIF4G1(1-1023)-SalI. (W303 strain as a genomic template). Expresses eIF4G1 N' terminus fused to GAL4AD
Proper citation: RRID:Addgene_196833 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MCP-2xGFP
Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36357695
Proper citation: RRID:Addgene_197047 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4573; Vector Backbone:HO-poly-KanMX4-HO (ATCC 87804); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36849832
Comments: Supplementary Table 2 of the paper provides details on the cloning of the plasmid.
Proper citation: RRID:Addgene_196616 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PAH1
Vector Backbone Description: Vector Backbone:UG75; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32859196
Proper citation: RRID:Addgene_198597 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LifeAct-eGFP
Vector Backbone Description: Backbone Size:5103; Vector Backbone:PZac2.1; Vector Types:Mammalian Expression, Bacterial Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37046092
Proper citation: RRID:Addgene_190199 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pCAG-FSF-LSL
Vector Backbone Description: Backbone Size:6249; Vector Backbone:pBT378; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36220098
Proper citation: RRID:Addgene_197044 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mTagBFP2:S. cerevisiae Mss4(377-756)(D636K)
Vector Backbone Description: Vector Backbone:pTagBFP2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37534432
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.30.498262v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_202725 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756)
Vector Backbone Description: Vector Backbone:piRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37534432
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.30.498262v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_202744 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Size:7400; Vector Backbone:MTTH-EGFP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36972455
Comments: A289V mutation in Dcp2 insert wil not affect plasmid function.
Proper citation: RRID:Addgene_201425 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: XI1_DW
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198684 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: XI1_UP
Vector Backbone Description: Backbone Marker:IndBioTech Lab; Vector Backbone:pGA-blue; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37058502
Comments: Cloning Method: Goldon Gate Assembly
Proper citation: RRID:Addgene_198683 Copy
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