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Species: Caenorhabditis elegans
Genetic Insert: cxTi10816 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34864 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4391 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34869 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34868 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10882 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34867 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 region
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Unpublished MosSCI reagents from the Jorgensen lab.
This vector targets the site ttTi4348 on Chr. I.
Use with strain EG6701 at the CGC.
Note - plasmid contains TWO M13F primer sites.
Proper citation: RRID:Addgene_30538 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 region
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pDESTR4-R3 derivative; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30539 Copy
Species: Caenorhabditis elegans
Genetic Insert: npp-16
Vector Backbone Description: Backbone Marker:Caldwell(?); Backbone Size:2790; Vector Backbone:pL4440gw; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20335358
Proper citation: RRID:Addgene_37495 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2::fbf-2 3’UTR
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6700; Vector Backbone:pEV1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20335358
Proper citation: RRID:Addgene_37498 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7066; Vector Backbone:MXW-PGK-IRES-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22142974
Proper citation: RRID:Addgene_37645 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ub-M
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37841 Copy
Species: Caenorhabditis elegans
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37833 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnhx-2F13D12.6::YFP
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:3410; Vector Backbone:pPD49.26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22768338
Proper citation: RRID:Addgene_37836 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR)
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid pPK605 contains two inserts: (1) unc-119 promoter, unc-119 and unc-119 3'UTR cassette and (2) pie-1 promoter, MluI/BamHI cloning site and pie-1 3'UTR
Please see plasmid map link above for more details
Proper citation: RRID:Addgene_38148 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans glutamate-gated chloride channel alpha, crystallized construct
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pGEM-HE, modified; Vector Types:For RNA synthesis for expression in X. laevis oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21572436
Comments: For vector information, see: Liman et al. 1992 Neuron 9:861-871.
For RNA synthesis, linearize plasmid with NheI.
Proper citation: RRID:Addgene_31487 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16a specific
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Comments: Contains only the isoform A-specific region
Proper citation: RRID:Addgene_31503 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16d/f specific
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Comments: Please note that Addgene's sequencing results found a single nucleotide mismatch at bp# 3 when compared to the sequence provided by the depositing scientist. This mismatch is not a concern for the function of the plasmid
Proper citation: RRID:Addgene_31505 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16a/b/f common
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613724
Proper citation: RRID:Addgene_31507 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-12
Vector Backbone Description: Backbone Size:3791; Vector Backbone:pCMV myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21814518
Proper citation: RRID:Addgene_32566 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Size:2131; Vector Backbone:pGOv5; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23281894
Proper citation: RRID:Addgene_42164 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Size:2131; Vector Backbone:pGOv5; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23281894
Proper citation: RRID:Addgene_42165 Copy
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