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Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71501 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71505 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: We replaced the SV40 promotor of the pGL3-promotor plasmid by DSCP and inserted a Gateway-cassette into the multiple-cloning-site (MCS; using KpnI and BglII), i.e. upstream of the DSCP, to allow Gateway cloning of candidate sequences
Proper citation: RRID:Addgene_71506 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The original human STARR-seq plasmid has been used in the Arnold et al. 2013 publication. It should be replaced for all screens in mammalian cells with the updated version reported by Muerdter et al. 2017 for much improved signal-to-noise, especially for screens with highly complex libraries (e.g. those covering an entire mammalian genome). The improved version is Addgene plasmid # 99296. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).
The sequence between SacI and AfeI on pGL4 was replaced by the fragment containing the Super Core Promoter 1 (SCP1), a synthetic intron (pIRESpuro3, Clontech; cat. no. 631619) an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms, and the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71509 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:16320; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16499902
Comments: Use ONLY DB3.1 to transform this plasmid. DH5 alpha does not work (it has a death gene).
Proper citation: RRID:Addgene_22720 Copy
Species: artifical
Genetic Insert: Gateway-SnAvi
Vector Backbone Description: Backbone Marker:pTriEx5 (Novagen); Backbone Size:6027; Vector Backbone:pBY2887; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20047968
Proper citation: RRID:Addgene_23222 Copy
Genetic Insert: TEV protease, S219V mutant
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:11809930
Comments: Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene).
pRK793 overproduces the catalytic domain of tobacco etch virus (TEV) protease in the form of an MBP fusion protein that cleaves itself in vivo to yield a TEV protease catalytic domain with an N-terminal His-tag and a C-terminal polyarginine tag.
Proper citation: RRID:Addgene_8827 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6700; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:12606030
Comments: A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of V. cholera maltose-binding protein. Presumptive N-terminal signal peptide deleted.
Proper citation: RRID:Addgene_8841 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5675; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:12606030
Comments: A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of Yersinia pestis maltose-binding protein.
Proper citation: RRID:Addgene_8836 Copy
Species: tobacco etch virus
Genetic Insert: tobacco etch virus protease, catalytic domain
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:11809930
Comments: pRK1043 overproduces the catalytic domain of tobaco etch virus (TEV) protease in the form of an affinity sandwich, with E. coli maltose-binding protein fused to its N-terminus and a polyarginine tag fused to its C-terminus. TheTEV protease produced by this strain is the autoinactivation-resistant mutant S219V.The pRIL plasmid (Stratagene) improves the yield of TEV protease by increasing the supply of argU tRNA in the cell (for AGG and AGA codons). The catalytic activity of the S219V mutant is approximately 2-fold greater than that of the wild-type protease. Shifting the temperature from 37°C to 30°C during induction maximizes the yield of soluble MBP-TEV-Arg fusion protein. Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene).
Proper citation: RRID:Addgene_8835 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6700; Vector Backbone:pET11; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:12606030
Comments: A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of P. furiosus maltose-binding protein. Lone cysteine residue mutated to serine. Presumptive N-terminal signal peptide deleted.
Proper citation: RRID:Addgene_8838 Copy
Genetic Insert: TEV protease, S219P mutant
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:11809930
Comments: pRK792 overproduces the catalytic domain of tobbaco etch virus (TEV) protease in the form of an MBP fusion protein that cleaves itself in vivo to yield a TEV protease catalytic domain with an N-terminal His-tag and a C-terminal polyarginine tag. The TEV protease produced by this strain is the autoinactivation-resistant mutant S219P.The pRIL plasmid (Stratagene) improves the yield of TEV protease by increasing the supply of argU tRNA in the cell (for AGG and AGA codons). The catalytic activity of the S219P mutant is approximately 2-fold less than that of the wild-type protease. Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene).
Proper citation: RRID:Addgene_8830 Copy
Vector Backbone Description: Vector Backbone:pTrcHis2B; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30788784
Proper citation: RRID:Addgene_88894 Copy
Species: Danio rerio
Genetic Insert: zic2a
Vector Backbone Description: Backbone Marker:Nadav Ahituv lab; Vector Backbone:E1b-GFP-Tol2-Gateway; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27965291
Proper citation: RRID:Addgene_89381 Copy
Species: Mus musculus
Genetic Insert: Irf2bpl
Vector Backbone Description: Backbone Marker:Nadav Ahituv lab; Vector Backbone:E1b-GFP-Tol2-Gateway; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27965291
Proper citation: RRID:Addgene_89380 Copy
Species: Danio rerio
Genetic Insert: zic2a
Vector Backbone Description: Backbone Marker:Nadav Ahituv lab; Vector Backbone:E1b-GFP-Tol2-Gateway; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27965291
Proper citation: RRID:Addgene_89382 Copy
Species: Danio rerio
Genetic Insert: zic2a
Vector Backbone Description: Backbone Marker:Nadav Ahituv lab; Vector Backbone:E1b-GFP-Tol2-Gateway; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27965291
Proper citation: RRID:Addgene_89388 Copy
Species: Danio rerio
Genetic Insert: irf2bpl
Vector Backbone Description: Backbone Marker:Nadav Ahituv lab; Vector Backbone:E1b-GFP-Tol2-Gateway; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27965291
Proper citation: RRID:Addgene_89370 Copy
Species: Mus musculus
Genetic Insert: Irf2bpl
Vector Backbone Description: Backbone Marker:Nadav Ahituv lab; Vector Backbone:E1b-GFP-Tol2-Gateway; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27965291
Proper citation: RRID:Addgene_89374 Copy
Species: Danio rerio
Genetic Insert: irf2bpl
Vector Backbone Description: Backbone Marker:Nadav Ahituv lab; Vector Backbone:E1b-GFP-Tol2-Gateway; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27965291
Proper citation: RRID:Addgene_89372 Copy
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