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Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Size:3740; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29123102
Proper citation: RRID:Addgene_107369 Copy
Species: Rattus norvegicus
Genetic Insert: calcium channel beta1b auxillary subunit
Vector Backbone Description: Backbone Marker:Genetics Institute, Inc, Cambridge, MA, USA; Backbone Size:5163; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9006976
Comments: Please note, although not a perfect match, the nucleotide sequence of this plasmid most closely matches NM_017346.1 Rattus norvegicus cacnb1 mRNA.
Proper citation: RRID:Addgene_107423 Copy
Species: Rattus norvegicus
Genetic Insert: cacnb4
Vector Backbone Description: Backbone Marker:Genetics Institute, Inc, Cambridge, MA, USA; Backbone Size:5163; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11756448
Comments: We received it in pBluescript and subcloned it into pMT2 plasmid.
Proper citation: RRID:Addgene_107426 Copy
Species: Rattus norvegicus
Genetic Insert: VGF promoter
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1570299
Proper citation: RRID:Addgene_107574 Copy
Species: Rattus norvegicus
Genetic Insert: VGF8a
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3839317
Comments: Note: The VGF insert contains a V480M mutation compared to the closest reference sequence. The depositor is unable to confirm what, if any, functional impact this has on the plasmid.
Proper citation: RRID:Addgene_107572 Copy
Species: Rattus norvegicus
Genetic Insert: synaptophysin-GCaMP6s, mRuby3
Vector Backbone Description: Backbone Marker:Bong-Kiun Kaang; Backbone Size:4476; Vector Backbone:pAAV-CW3SL-EGFP; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This plasmid contains a naturally-occurring a G→T mutation in one of the AAV ITRs at position 570bp. However, we have still obtained high titer AAV using this plasmid and robust in vivo expression in Cre+ mouse neurons.
Proper citation: RRID:Addgene_107737 Copy
Species: Rattus norvegicus
Genetic Insert: synaptophysin-GCaMP6s, mRuby3
Vector Backbone Description: Backbone Marker:Bong-Kiun Kaang; Backbone Size:3372; Vector Backbone:pAAV-CW3SL-EGFP; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107736 Copy
Species: Rattus norvegicus
Genetic Insert: PKC zeta
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9768361
Proper citation: RRID:Addgene_10800 Copy
Species: Rattus norvegicus
Genetic Insert: PKC zeta
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9768361
Comments: Constitutively active. Myr sequence from p60src. Note: There is a HindIII site in the insert ~1100bp from the 5'end.
Proper citation: RRID:Addgene_10802 Copy
Species: Rattus norvegicus
Genetic Insert: rLAMP1
Vector Backbone Description: Backbone Marker:Jacob Corn ; Backbone Size:7860; Vector Backbone:unknown; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30143524
Proper citation: RRID:Addgene_109015 Copy
Species: Rattus norvegicus
Genetic Insert: neuT
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pSV2-dhfr; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2871941
Comments: See author's map. Expressed in NIH cells, but pSV2 neuNT expresses better.
Proper citation: RRID:Addgene_10919 Copy
Species: Rattus norvegicus
Genetic Insert: anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29915061
Proper citation: RRID:Addgene_109419 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII(C450A)-ShadowG
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_167479 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4682; Vector Backbone:pmRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28320970
Proper citation: RRID:Addgene_167970 Copy
Species: Rattus norvegicus
Genetic Insert: Rfz1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96.
Rfz1 from Henk Roelink. This sequence has a few differences
from the published sequence. Sites listed in Rfz1 are not a complete list. MYC tag version made by Jeff Brown (2/98-3/98), inserted the epitope into the StyI site (1904). The MYC epitope has a BglII site in it. The 3'
Sty site is destroyed by the tags, but the 5' site is intact. Epitope can be detected in PBS/Formaldehyde fixed tissue permeabilized with 0.2% Triton.
The Myc tagged Rfz1 has been IP'd from a TBS/1% Triton buffer. Frizzled tends to ppt out of SDS sample buffer when boiled, so either warm samples
to ~70 degrees Celsius for a few minutes before loading samples or use a strong denaturing loading buffer and incubate the samples at room temp for half an hour before loading. The StyI site is in the 3rd extracellular loop of the protein.
Proper citation: RRID:Addgene_16798 Copy
Species: Rattus norvegicus
Genetic Insert: Rfz2 TG2myc
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Jeff Brown (tags), 11/23/98.
A single c-myc epitope has been inserted into the third extracellular loop of Rfz2 by introduction of an NheI site and the epitope. The epitope
was inserted in the region of the loop where the myc and the HA tags were inserted into the Rfz1 tagged constructs--3 aa's after the PCP sequence--which
appear to be functional.
*The ClaI and XbaI sites are methylated and cannot be cut unless the construct is grown up in dam-bugs.
Proper citation: RRID:Addgene_16796 Copy
Species: Rattus norvegicus
Genetic Insert: beta 2 AR Fz1
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pCDNA3.1; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588714
Comments: Chimera of beta 2 adrenergic receptor (extracellular and.
transmembrane domains) and rat frizzled-1 (intracellular). Activated by isoproterenol and inhibited by propranolol.
Ref: X. Lui et al., PNAS, 1999, 96:14383-14388 and M. Kuehl et al.,
2000, JBC 275:12701-12711.
Proper citation: RRID:Addgene_16795 Copy
Species: Rattus norvegicus
Genetic Insert: rat frizzled 2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Insert size: ~1912 bp (EcoRI/Xba I both blunted)
Transcription: linearize with Asp 718 and transcribe with SP6.
Plasmid History:
Constructed by Julia Yang-Snyder on the date of 5/19/96.
Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306,
1996.
Proper citation: RRID:Addgene_16814 Copy
Species: Rattus norvegicus
Genetic Insert: rat frizzled 1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Transcription: linearize with Not I or Asp 718 and transcribe with SP6.
Plasmid History:
Constructed by Julia Yang-Snyder on the date of 4/29/96.
Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.
Proper citation: RRID:Addgene_16821 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-1 cDNA was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The insert was published by Wang et al., JBC 271: 4468 (1996). This unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16820 Copy
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