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Species: Rattus norvegicus
Genetic Insert: Erk2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24704509
Proper citation: RRID:Addgene_40777 Copy
Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11591711
Proper citation: RRID:Addgene_40820 Copy
Species: Rattus norvegicus
Genetic Insert: LAP-NLG1
Vector Backbone Description: Backbone Size:6389; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23457442
Proper citation: RRID:Addgene_43924 Copy
Species: Rattus norvegicus
Genetic Insert: APOBEC-nCas9-rXRCC1
Vector Backbone Description: Backbone Marker:origene; Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33654077
Proper citation: RRID:Addgene_165444 Copy
Species: Rattus norvegicus
Genetic Insert: APOBEC-nCas9-rPB(8kD)
Vector Backbone Description: Backbone Marker:origene; Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33654077
Proper citation: RRID:Addgene_165445 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII(T286A)
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_165439 Copy
Species: Rattus norvegicus
Genetic Insert: FHS-paCaMKII
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Proper citation: RRID:Addgene_165429 Copy
Species: Rattus norvegicus
Genetic Insert: iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain
Vector Backbone Description: Backbone Size:4753; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36622100
Comments: Chimera of iGluSnFR (Addgene plasmid # 41732), Neto2 and Gamma-2
pAAV backbone is based on Addgene #61463
Please visit https://doi.org/10.1101/2021.01.21.427382 for bioRxiv preprint.
Proper citation: RRID:Addgene_165497 Copy
Species: Rattus norvegicus
Genetic Insert: iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5460; Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36622100
Comments: Chimera of iGluSnFR (Addgene plasmid # 41732), Neto2 and Gamma-2 (Stargazin).
Please visit https://doi.org/10.1101/2021.01.21.427382 for bioRxiv preprint.
Proper citation: RRID:Addgene_165495 Copy
Species: Rattus norvegicus
Genetic Insert: iGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5460; Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36622100
Comments: Chimera of iGluSnFR (Addgene plasmid # 41732), Neto2 and Gamma-8.
Please visit https://doi.org/10.1101/2021.01.21.427382 for bioRxiv preprint.
Proper citation: RRID:Addgene_165496 Copy
Species: Rattus norvegicus
Genetic Insert: CAMKII-K42M 1-271
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777564
Comments: Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.
Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.
Proper citation: RRID:Addgene_16734 Copy
Species: Rattus norvegicus
Genetic Insert: CaM Kinase II
Vector Backbone Description: Backbone Marker:Maurer lab; Backbone Size:4265; Vector Backbone:pRSV-link; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7958915
Proper citation: RRID:Addgene_45065 Copy
Species: Rattus norvegicus
Genetic Insert: Kif 1a
Vector Backbone Description: Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16543128
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_45058 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060023
Proper citation: RRID:Addgene_45449 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060023
Proper citation: RRID:Addgene_45448 Copy
Species: Rattus norvegicus
Genetic Insert: NR2B
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Comments: Addgene Next-generation sequencing identified a sequence discrepancy that results in a Met to Leu change at position 745 in the plasmid insert, relative to Genbank IDs NM_012574.1 and NP_036706.1. The plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_45447 Copy
Species: Rattus norvegicus
Genetic Insert: Plasminogen activator inhibitor type 1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3149611
Proper citation: RRID:Addgene_45389 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta tagged by mEGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45523 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45522 Copy
Species: Rattus norvegicus
Genetic Insert: rM3D (Gs)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5026; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360345
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_45549 Copy
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