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Vector Backbone Description: Vector Backbone:pLIX403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: 5' Cloning Site: PacI (not destroyed), 3' Cloning Site: SbfI (not destroyed)
Proper citation: RRID:Addgene_158564 Copy
Vector Backbone Description: Vector Backbone:pLIX403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: 5' Cloning Site: PacI (not destroyed), 3' Cloning Site: SbfI (not destroyed)
Proper citation: RRID:Addgene_158561 Copy
Vector Backbone Description: Vector Backbone:pLIX403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: 5' Cloning Site: PacI (not destroyed), 3' Cloning Site: SbfI (not destroyed)
Proper citation: RRID:Addgene_158562 Copy
Vector Backbone Description: Vector Backbone:pLIX403; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_158560 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike protein
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35123472
Proper citation: RRID:Addgene_171639 Copy
Species: Mus musculus
Genetic Insert: ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
Vector Backbone Description: Vector Backbone:pEF1a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33972728
Comments: The tBE system with gRNA could be constructed by linearing pU6-ccdB-boxB-tBE-V5-mA3 (backbone vector of tBE system) and inserting the gRNA sequences amplificating from pUC57-sgRNA-MS2-U6. The detailed protocol could be found in Supplementary Protocol in the publication.
Proper citation: RRID:Addgene_171693 Copy
Species: Thermatoga maritima
Genetic Insert: Cellulose Bindind Protein 9
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35123472
Proper citation: RRID:Addgene_171708 Copy
Species: Other
Genetic Insert: LSL-Tomato
Vector Backbone Description: Vector Backbone:Rosa26; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26018130
Proper citation: RRID:Addgene_66590 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26658467
Proper citation: RRID:Addgene_66937 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: attR1-ccdB-CamR-attR2-eGFP-8xHis-Stop
Proper citation: RRID:Addgene_67138 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: attR1-ccdB-CamR-attR2-mCheery-8xHis-Myc-Stop
Proper citation: RRID:Addgene_67142 Copy
Vector Backbone Description: Vector Backbone:pCellFree_G08; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: sfGFP-8xHis
Proper citation: RRID:Addgene_67144 Copy
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338
Proper citation: RRID:Addgene_67616 Copy
Vector Backbone Description: Backbone Size:4181; Vector Backbone:PCS2+ was modified to generate pCSf107mT; Vector Types:Mammalian Expression, in vitro transcription or translation off of an SP6 promoter, Xenopus expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26391338
Proper citation: RRID:Addgene_67617 Copy
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27621059
Proper citation: RRID:Addgene_67691 Copy
Species: Synthetic
Genetic Insert: mCherry-attR1/attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: Please note that mutation H30R in mCherry was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_73487 Copy
Species: Synthetic
Genetic Insert: fli1ep
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73486 Copy
Species: Other
Genetic Insert: 6xmyc/attR1/attR2
Vector Backbone Description: Vector Backbone:pTol2005b; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73485 Copy
Species: Synthetic
Genetic Insert: fli1ep:EGFP-attR1-attR2
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_73491 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5439; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27405979
Proper citation: RRID:Addgene_73637 Copy
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