Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Genetic Insert: 6x Myc Tag
Vector Backbone Description: Backbone Size:5818; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13070 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:10365; Vector Backbone:pGAN049; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is one of the empty dual yTRAP sensor plasmids. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK247. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with pSK260.
Proper citation: RRID:Addgene_131148 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:11381; Vector Backbone:pGAN147; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty reverse yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN230. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin. To achieve dual color reporting, combine with pSK221.
Proper citation: RRID:Addgene_131122 Copy
Vector Backbone Description: Backbone Marker:Szilvia Kiriakov; Backbone Size:10377; Vector Backbone:pSK247; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty dual yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK234. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with an expression clone resulting from LR reaction using pGAN147 basic yTRAP sensor.
Proper citation: RRID:Addgene_131149 Copy
Species: Synthetic
Genetic Insert: PGK-puroR-pA cassette, inserted in PB-TET vector
Vector Backbone Description: Backbone Marker:Andras Nagy (Addgene plasmid # 20909); Backbone Size:13983; Vector Backbone:PB-TET (addgene #20909); Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_139561 Copy
Vector Backbone Description: Backbone Marker:Linzhao Cheng (Addgene plasmid # 28213); Backbone Size:9114; Vector Backbone:pEB-C5 (Addgene #28213); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone
Proper citation: RRID:Addgene_139519 Copy
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pCAGGS; Vector Types:Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted between loxP
Proper citation: RRID:Addgene_139517 Copy
Vector Backbone Description: Backbone Marker:Linzhao Cheng (Addgene plasmid # 28213); Backbone Size:11319; Vector Backbone:pEB-C5 (Addgene #28213); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone
Proper citation: RRID:Addgene_139520 Copy
Vector Backbone Description: Backbone Size:4338; Vector Backbone:pMC1DT-ApA; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone; Uses Diphtheria toxin A (DTA) as negative selection.
Proper citation: RRID:Addgene_139521 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7892; Vector Backbone:pHTN HaloTag CMV-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_136403 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7662; Vector Backbone:pNLF1-N CMV-hygro; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_136404 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4720; Vector Backbone:pQE-2; Vector Types:Bacterial Expression, Co-Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17311810
Comments: Please note that the chloramphenicol resistance gene outside of the attB sites in this plasmid is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.
Proper citation: RRID:Addgene_13668 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:5356; Vector Backbone:pQE-2; Vector Types:Bacterial Expression, Co-Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17311810
Comments: Please note that the chloramphenicol resistance gene outside the attB sites in this plasmid is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.
Proper citation: RRID:Addgene_13673 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6332; Vector Backbone:T-Vector pMD19 (Simple); Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31740773
Comments: This plasmid is designed for transient transfection of protoplasts. It has only been validated in Zea mays mesophyll protoplasts. The region containing the ccdB suicide gene is dropped out by a double-digest with SacI and KpnI and the large fragment is recombined with the DNA insert library.
Proper citation: RRID:Addgene_117379 Copy
Species: Fluorescent protein genes from jellyfish and coral
Genetic Insert: Brainbow 1.0 L - ccdB survival cassette
Vector Backbone Description: Vector Backbone:pT2-Ip2GI; Vector Types:zebrafish expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23757414
Proper citation: RRID:Addgene_118221 Copy
Vector Backbone Description: Backbone Size:7509; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24292623
Proper citation: RRID:Addgene_101139 Copy
Vector Backbone Description: Vector Backbone:pMH-HA-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28325877
Proper citation: RRID:Addgene_101766 Copy
Vector Backbone Description: Vector Backbone:pMH-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28325877
Proper citation: RRID:Addgene_101767 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike protein
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35123472
Proper citation: RRID:Addgene_171639 Copy
Species: Mus musculus
Genetic Insert: ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
Vector Backbone Description: Vector Backbone:pEF1a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33972728
Comments: The tBE system with gRNA could be constructed by linearing pU6-ccdB-boxB-tBE-V5-mA3 (backbone vector of tBE system) and inserting the gRNA sequences amplificating from pUC57-sgRNA-MS2-U6. The detailed protocol could be found in Supplementary Protocol in the publication.
Proper citation: RRID:Addgene_171693 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
