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Species: Saccharomyces cerevisiae
Genetic Insert: pYPS1-YFP
Vector Backbone Description: Vector Backbone:pYTK143; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32898473
Proper citation: RRID:Addgene_133118 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153106 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153101 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: α Galactosidase SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153504 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Invertase 2 SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153493 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Prepro SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL11B
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPS1530; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11071906
Comments: CEN vector with ORF of GFP followed by the 3'UTR of NUF2.
Proper citation: RRID:Addgene_1364 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PGK1-U1A-PGK1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: PGK1 promoter and PGK1 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), PGK1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1370 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL25-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: RPL25 promoter and RPL25 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1369 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA4-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: SSA4 promoter and SSA4 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1371 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PGK1-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: PGK1 promoter and PGK1 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1372 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5332; Vector Backbone:pET28a-derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Proper citation: RRID:Addgene_13729 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5312; Vector Backbone:pET28a derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Comments: Construct is expressed with a precission protease cleavable 6xHis tag
Proper citation: RRID:Addgene_13727 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeTadA1.0-T7 RNAP
Vector Backbone Description: Backbone Size:5600; Vector Backbone:ColE1/AMP; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33707425
Comments: Depositor confirms E366G does not affect plasmid function.
Proper citation: RRID:Addgene_137735 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL25-U1A-PGK1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: RPL25 promoter and RPL25 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), PGK1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1375 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NPL3
Vector Backbone Description: Backbone Size:5181; Vector Backbone:YEp352; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8675010
Comments: ScaI fragment of NPL3 cloned into YEp352 SmaI site.
Proper citation: RRID:Addgene_1378 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal-Clb2-GFP
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pPS1304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11171327
Comments: pPS1304 has the GAL1 promoter (EcoRI/XbaI fragment) and GFP (HindIII fragment) in YEp352 (2 micron ori).
Proper citation: RRID:Addgene_1381 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sti1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pUGPD (pRS316 carrying GPD promoter, based on pBluescript); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8972212
Comments: Plasmid maintained in DH5alpha, CEN and selectable URA3 marker. Full-length Sti1 gene, expressed at level comparable to the endogenous gene.
Proper citation: RRID:Addgene_1335 Copy
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