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pT-77
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_16087 ull1 delta440 Saccharomyces cerevisiae Ampicillin PMID:12761287 Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:45 1
pLNL1133
 
Resource Report
Resource Website 		RRID:Addgene_160864 Medium-chain fatty acid ethyl ester synthase Saccharomyces cerevisiae Kanamycin PMID:32886882 Vector Backbone:p15A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-09-01 09:25:45 0
pT-35
 
Resource Report
Resource Website 		RRID:Addgene_16092 SMT3 Saccharomyces cerevisiae Ampicillin PMID:11577116 Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:46 0
pT-60
 
Resource Report
Resource Website 		RRID:Addgene_16090 UBC9 Saccharomyces cerevisiae Ampicillin PMID:12761287 Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:45 0
pGST-pol1-N1-2A2
 
Resource Report
Resource Website 		RRID:Addgene_160990 pol1 Saccharomyces cerevisiae Ampicillin PMID:32923642 Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin F58A/D62A 2026-09-01 09:25:47 0
YIplac211-sGFP-Vrg4
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_160904 VRG4 Saccharomyces cerevisiae Ampicillin PMID:30858194 Parental strain JK93da. Backbone Size:4000; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:45 1
pGST-pol1-N1
 
Resource Report
Resource Website 		RRID:Addgene_160988 pol1 Saccharomyces cerevisiae Ampicillin PMID:32923642 Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:47 0
plIVMD163
 
Resource Report
Resource Website 		RRID:Addgene_160935 MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop Saccharomyces cerevisiae Ampicillin PMID:33037152 Backbone Size:6000; Vector Backbone:plIVMD156; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:46 0
plIVMD164
 
Resource Report
Resource Website 		RRID:Addgene_160936 MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop Saccharomyces cerevisiae Ampicillin PMID:33037152 Please note that the T247A mutation in sfGFP does not impact plasmid function. Backbone Size:6000; Vector Backbone:plIVMD156; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:46 0
plIVMD217
 
Resource Report
Resource Website 		RRID:Addgene_160939 MCP-mTagBFP2-HA Tag Saccharomyces cerevisiae Ampicillin PMID:33037152 Vector Backbone:psh100; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:25:46 0
pSMH1741
 
Resource Report
Resource Website 		RRID:Addgene_160930 HHF1 Saccharomyces cerevisiae Ampicillin This is an improved version of the plasmid reported here: https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X https://doi.org/10.1016/j.str.2019.12.002 All histone genes code for S. cerevisiae proteins. Expression and purification procedures are as described. Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin codon-optimized for E. coli -- see provided sequence 2026-09-01 09:25:46 0
pSMH1742
 
Resource Report
Resource Website 		RRID:Addgene_160929 HHF1 Saccharomyces cerevisiae Ampicillin This is an improved version of the plasmid reported here: https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X https://doi.org/10.1016/j.str.2019.12.002 All histone genes code for S. cerevisiae proteins. Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin codon-optimized for E. coli -- see provided sequence 2026-09-01 09:25:46 0
pCAG-FlpeERT2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_14756 Flpe-ERT2 fusion protein Saccharomyces cerevisiae Ampicillin PMID:17209010 Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation. Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin 2026-09-01 09:22:28 3
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)
 
Resource Report
Resource Website 		RRID:Addgene_14841 PHO5 promoter UASm1 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin UASm1 (AAGCTT was substituted for CACGTT). Uninducible promoter. See Steger DJ et al Science 299(5603)114-116. 2026-09-01 09:22:46 0
pJRL2 GAL1pr VYFP (EB#1571)
 
Resource Report
Resource Website 		RRID:Addgene_14847 GAL1 promoter Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:22:48 0
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)
 
Resource Report
Resource Website 		RRID:Addgene_14852 GAL1 promoter TATA-C2 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-C2. TATA box mutated from TATATAAG to TCTATAAG. 2026-09-01 09:22:49 0
pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)
 
Resource Report
Resource Website 		RRID:Addgene_14851 GAL1 promoter TATA-C2 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-C2. TATA box mutated from TATATAAG to TCTATAAG. 2026-09-01 09:22:49 0
pGBW-m4046278
 
Resource Report
Resource Website 		RRID:Addgene_149000 SARS-CoV-2 ORF6 protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724394.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-09-01 09:22:59 0
pGBW-m4046305
 
Resource Report
Resource Website 		RRID:Addgene_149004 SARS-CoV-2 helicase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725308.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-09-01 09:22:59 0
pGBW-m4046454
 
Resource Report
Resource Website 		RRID:Addgene_149006 SARS-CoV-2 RNA-dependent RNA polymerase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-09-01 09:22:59 0

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