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http://www.addgene.org/59929

Species: Caenorhabditis elegans
Genetic Insert: rde-1(H974) gRNA
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212

Proper citation: RRID:Addgene_59929 Copy   

•   Source: Addgene

http://www.addgene.org/63138

Species: Caenorhabditis elegans
Genetic Insert: Tc3A
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET3C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15304566

Proper citation: RRID:Addgene_63138 Copy   

•   Source: Addgene

http://www.addgene.org/62599

Species: Caenorhabditis elegans
Genetic Insert: Thr412Gly-CePheRS Alpha Subunit
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid #1494) ; Backbone Size:4494; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25691744

Proper citation: RRID:Addgene_62599 Copy   

•   Source: Addgene

http://www.addgene.org/62867

Species: Caenorhabditis elegans
Genetic Insert: MBP-PGL-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836

Proper citation: RRID:Addgene_62867 Copy   

•   Source: Addgene

http://www.addgene.org/62869

Species: Caenorhabditis elegans
Genetic Insert: MBP-PPTR-2
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836

Proper citation: RRID:Addgene_62869 Copy   

•   Source: Addgene

http://www.addgene.org/62865

Species: Caenorhabditis elegans
Genetic Insert: PAA-1/Y3H
Vector Backbone Description: Backbone Size:8128; Vector Backbone:pY3H Gateway compatible destination vector; Vector Types:Yeast Expression, two-hybrid bridge vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836

Proper citation: RRID:Addgene_62865 Copy   

•   Source: Addgene

http://www.addgene.org/62879

Species: Caenorhabditis elegans
Genetic Insert: GST-MEG-1
Vector Backbone Description: Backbone Size:4984; Vector Backbone:pGEX6p1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836

Proper citation: RRID:Addgene_62879 Copy   

•   Source: Addgene

http://www.addgene.org/62875

Species: Caenorhabditis elegans
Genetic Insert: MBP-MEG-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836

Proper citation: RRID:Addgene_62875 Copy   

•   Source: Addgene

http://www.addgene.org/62876

Species: Caenorhabditis elegans
Genetic Insert: MBP-MEG-3
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836

Proper citation: RRID:Addgene_62876 Copy   

•   Source: Addgene

http://www.addgene.org/62872

Species: Caenorhabditis elegans
Genetic Insert: MBP-MBK-2 K196R
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836

Proper citation: RRID:Addgene_62872 Copy   

•   Source: Addgene

http://www.addgene.org/62881

Species: Caenorhabditis elegans
Genetic Insert: GST-MEG-4
Vector Backbone Description: Backbone Size:4984; Vector Backbone:pGEX6p1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836

Proper citation: RRID:Addgene_62881 Copy   

•   Source: Addgene

http://www.addgene.org/62882

Species: Caenorhabditis elegans
Genetic Insert: GFP::MEG-3
Vector Backbone Description: Backbone Marker:Seydoux lab, Addgene plasmid # 26955; Backbone Size:8260; Vector Backbone:pID2.02; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Comments: InFusion cloning

Proper citation: RRID:Addgene_62882 Copy   

•   Source: Addgene

http://www.addgene.org/51868

Species: Caenorhabditis elegans
Genetic Insert: psdhb-1::mtLS-cpYFP
Vector Backbone Description: Backbone Marker:B. D. Lemire; Backbone Size:3470; Vector Backbone:pSDH2(P-2)GFP; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24522532

Proper citation: RRID:Addgene_51868 Copy   

•   Source: Addgene

http://www.addgene.org/52033

Species: Caenorhabditis elegans
Genetic Insert: Unc-43
Vector Backbone Description: Vector Backbone:pGEX-6P; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23805378

Proper citation: RRID:Addgene_52033 Copy   

•   Source: Addgene

http://www.addgene.org/52513

Species: Caenorhabditis elegans
Genetic Insert: Dre-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5404; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396

Proper citation: RRID:Addgene_52513 Copy   

•   Source: Addgene

http://www.addgene.org/52514

Species: Caenorhabditis elegans
Genetic Insert: blmp-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5421; Vector Backbone:pcDNA3.1-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24613396

Proper citation: RRID:Addgene_52514 Copy   

•   Source: Addgene

http://www.addgene.org/110883

Species: Caenorhabditis elegans
Genetic Insert: Pmec-4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5020; Vector Backbone:Gateway Destination Vector; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20203177

Proper citation: RRID:Addgene_110883 Copy   

•   Source: Addgene

http://www.addgene.org/110880

Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Backbone Size:2617; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16624912
Comments: Used StrataScript Reverse Transcriptase and a primer engineered with a restriction site to make the full length pde-4d cDNA (2025 bp). We then nicked the duplexed RNA with RNAse H and synthesize the 2nd strand of cDNA using E. coli DNA Polymerase I, which can do nick translation. The DNA Pol I uses the nicked RNA as primers to synthesize the second strand (it chews up the RNA in front of it and replaces with DNA). We then used Accuprime Pfx and primers engineered with restriction sites to amplify and clone the cDNA into Nhe I/ Age I cut KG#59 (rab-3:: expression vector; 4.9 Kb). Transformed into XL1-Blue electrocompetent cells. Qiagen miniprepped 2 clones to found those with correct insert, then submitted the 2 clones for sequence. Choose one clone that had correct sequence and made glycerol stock. Features of the expression construct: This expression construct has a promoter (rab-3) that will drive expression of the cDNA throughout the nervous system. This construct includes only the coding region of pde-4d, and the sequence is taken from the Wormbase WS134 release, gene designation R153.1d. This 'd' isoform seems to be the most common splice variant. The sequence AAAA is engineered just after the 5' restriction site and just before the initiator ATG. An AT immediately follows the TAA stop codon for a consensus stop site. Three introns (all located in untranslated regions of the vector) are present to give stable and uniform expression (1 in the 5' UTR, 1 just upstream of the unc-54 3' end, and 1 in the unc-54 3' end sequence). The vector includes the unc-54 3' untranslated end containing a poly A addition signal to stop transcription and provide a 3' UTR for the transcript. The vector also contains a "decoy" (see 1995 vector kit documentation) upstream of the promoter to reduce background expression in the gut and pharynx from read-through transcription.

Proper citation: RRID:Addgene_110880 Copy   

•   Source: Addgene

http://www.addgene.org/110881

Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Backbone Size:2617; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16624912
Comments: Started with KG#203 (contains pde-4d(+) coding region cDNA in rab-3:: expression vector) and use the QuikChange site directed mutagenesis method to introduce the D448N mutation. The mutation changes codon 448 of pde-4d from GAT(Asp) to AAT (Asn) (HDVDH to HNVDH). Miniprepped 4 clones and chose 2 with the correct size insert to sequence. Sequenced the entire insert of both clones, and kept one clone that has the correct mutation, but no other mutations. After sequencing, this insert was "re-cloned" into a fresh rab-3:: expression vector to ensure that there are no other vector mutations that could affect its performance or expression in transgenic worms. Features of the expression construct: This expression construct has a promoter (rab-3) that will drive expression of the cDNA throughout the nervous system. This construct includes only the 2025 bp coding region of pde-4d (and contains the D448N mutation), and the sequence is taken from the Wormbase WS134 release, gene designation R153.1d (the d isoform appears to be the most common based on analysis of EST cDNAs). The sequence AAAA is engineered just after the 5' restriction site and just before the initiator ATG. An AT immediately follows the TAA stop codon for a consensus stop site. Three introns (all located in untranslated regions of the vector) are present to give stable and uniform expression (1 in the 5' UTR, 1 just upstream of the unc-54 3' end, and 1 in the unc-54 3' end sequence). The vector includes the unc-54 3' untranslated end containing a poly A addition signal to stop transcription and provide a 3' UTR for the transcript. The vector also contains a "decoy" (see 1995 vector kit documentation) upstream of the promoter to reduce background expression in the gut and pharynx from read-through transcription.

Proper citation: RRID:Addgene_110881 Copy   

•   Source: Addgene

http://www.addgene.org/110875

Species: Caenorhabditis elegans
Genetic Insert: myo-3 promoter
Vector Backbone Description: Backbone Size:2617; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15489511
Comments: Note: Always streak out this culture for single colonies and do minipreps on >= 4 clones (~1/2 of colonies will be right). Choose small colonies after 18 - 20h growth. DNA yeild is low (~170 ng/ ul x 30 ul for a Qiagen 3 ml miniprep). Used Age I/ Xho I to cut out the 3800 bp acy-1 cDNA from KG#62 and cloned into the like-digested myo-3 expression vector pPD96.52 (6100 bp). Transform into XL1-Blue electrocompetent cells. Miniprep 4 clones to find one with correct size insert and vector and make glycerol stock and save the DNA. Features of the construct: This expression construct has a promoter (myo-3) that will drive expression of the acy-1 cDNA in the body wall muscle cells. The myo-3 promoter is generally active in body muscles (body wall, vulval, and intestine associated muscles including the anal depressor [Okkema et al., 1993 Genetics; Ardizzi and Epstein 1987 JCB]. The vector contains a "decoy" (see 1995 vector kit documentation) upstream of the promoter to reduce background expression in the gut and pharynx from read-through transcription. An unc-54 3' end containing a poly A addition signal is downstream of the MCS to stop transcription and provide a 3' UTR for the transcript. Three introns in the UTR's are included to give stable and uniform expression (1 in the 5' UTR, 1 just upstream of the unc-54 3' end, and 1 in the unc-54 3' end sequence). This construct includes only the coding region. The sequence AAAA is engineered just after the 5' restriction site and just before the initiator ATG. An AT immediately follows the TAA stop codon for a consensus stop site. Note: the acy-1 cDNA from which this clone was produced corresponds to the single acy-1 cDNA predicted by wormbase, which is F17C8.1

Proper citation: RRID:Addgene_110875 Copy   

•   Source: Addgene