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http://www.addgene.org/24636

Species: E. coli
Genetic Insert: 'tesA
Vector Backbone Description: Backbone Size:0; Vector Backbone:p15a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20111002
Comments: EJ Steen et al. 2009 Nature 10.1038/nature08721

Proper citation: RRID:Addgene_24636 Copy   

•   Source: Addgene

http://www.addgene.org/25435

Species: E. coli
Genetic Insert: reverse tetracycline-regulated transactivator advanced
Vector Backbone Description: Backbone Marker:unpublished; Backbone Size:7200; Vector Backbone:pMA2635rc; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19655272

Proper citation: RRID:Addgene_25435 Copy   

•   Source: Addgene

http://www.addgene.org/23336

Species: E. coli
Genetic Insert: Enhanced Circularly Permuted Outer Membrane Protein X
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18480093

Proper citation: RRID:Addgene_23336 Copy   

•   Source: Addgene

http://www.addgene.org/25846

Species: E. Coli
Genetic Insert: LplA-W37I
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Comments: The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.

Proper citation: RRID:Addgene_25846 Copy   

•   Source: Addgene

http://www.addgene.org/25845

Species: E. Coli
Genetic Insert: LplA-W37V
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Comments: The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.

Proper citation: RRID:Addgene_25845 Copy   

•   Source: Addgene

http://www.addgene.org/32296

Species: E. coli
Genetic Insert: lacZ ORF with short 5'UTR
Vector Backbone Description: Backbone Size:13150; Vector Backbone:pMS26; Vector Types:Bacterial Expression, bacterial gene addition; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22123741
Comments: Amplification and sequencing primers for verifying insertion into attTn7: glmS-proximal (Tn7R side) 5’-AATCTGTAACGTTCCGGGTTC-3’ OR 5'-GATGCTGGTGGCGAAGCTGT-3' pstS-proximal (Tn7L side) 5’-CATTAATAACGAAGAGATGAC-3’ OR 5'-GATGACGGTTTGTCACATGGA-3'

Proper citation: RRID:Addgene_32296 Copy   

•   Source: Addgene

http://www.addgene.org/32408

Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487

Proper citation: RRID:Addgene_32408 Copy   

•   Source: Addgene

http://www.addgene.org/33144

Species: E. coli
Genetic Insert: LacO (256 copies)
Vector Backbone Description: Backbone Marker:Addgene plasmid #33143; Vector Backbone:pVS1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18614049
Comments: Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable. For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142). pVS2 contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 [Addgene #33143] is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]). pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.

Proper citation: RRID:Addgene_33144 Copy   

•   Source: Addgene

http://www.addgene.org/33259

Species: E. coli
Genetic Insert: LexA::VP16-SV40 3'UTR
Vector Backbone Description: Vector Backbone:pCasPeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060186
Comments: A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006). The PCR fragment was then subcloned into the XbaI site of the pBS vector. LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter.

Proper citation: RRID:Addgene_33259 Copy   

•   Source: Addgene

http://www.addgene.org/27125

Species: E. coli
Genetic Insert: Colicin Ia + Ia immunity protein
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:PUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19919671
Comments: The original clone had some mutations that had been introduced during the PCR cloning of the colicin operon (into another vector). Here, the operon was moved into pUC19 and the mutations were corrected, so that the sequence is the same as the one in GenBank (M13819.1) This plasmid contains Colicin Ia + Ia immunity protein, each with their own promoters; colicin production can be induced with mitomycin C. The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other.

Proper citation: RRID:Addgene_27125 Copy   

•   Source: Addgene

http://www.addgene.org/27391

Species: E. coli
Genetic Insert: grpE
Vector Backbone Description: Backbone Size:4493; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9.

Proper citation: RRID:Addgene_27391 Copy   

•   Source: Addgene

http://www.addgene.org/27395

Species: E. coli
Genetic Insert: dnaK, dnaJ, groESL
Vector Backbone Description: Backbone Size:9177; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Between depositor sequence and Addgene sequencing results, there is a mismatch at position 7284 and an insertion at position 7360. These are in vector backbone and do not alter expression of the inserts. Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9.

Proper citation: RRID:Addgene_27395 Copy   

•   Source: Addgene

http://www.addgene.org/27396

Species: E. coli
Genetic Insert: dnaK, dnaJ, GroESL
Vector Backbone Description: Backbone Size:9203; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. single nt insertion at position 1726.

Proper citation: RRID:Addgene_27396 Copy   

•   Source: Addgene

http://www.addgene.org/27393

Species: E. coli
Genetic Insert: grpE, clpB
Vector Backbone Description: Backbone Size:7194; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9.

Proper citation: RRID:Addgene_27393 Copy   

•   Source: Addgene

http://www.addgene.org/21679

Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJH271; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1545810
Comments: The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and LacZ::CS cut site was inserted into the HindIII site at the upstream end of a promotorless LacZ gene on plasmid pJH271. The two LacZ sequences are inverted relative to eachother. The lambda sequences are of unknown origin Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.

Proper citation: RRID:Addgene_21679 Copy   

•   Source: Addgene

http://www.addgene.org/21673

Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ gene in inverted orientation, with one copy containing an HO endonuclease cleavage site. Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.

Proper citation: RRID:Addgene_21673 Copy   

•   Source: Addgene

http://www.addgene.org/21672

Species: E. coli
Genetic Insert: Lacz
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ gene in inverted orientation, with one copy containing an HO endonuclease cleavage site. Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.

Proper citation: RRID:Addgene_21672 Copy   

•   Source: Addgene

http://www.addgene.org/21671

Species: E. coli
Genetic Insert: Lacz
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ gene in inverted orientation, with one copy containing an HO endonuclease cleavage site. Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.

Proper citation: RRID:Addgene_21671 Copy   

•   Source: Addgene

http://www.addgene.org/21670

Species: E. coli
Genetic Insert: Lacz
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ gene in inverted orientation, with one copy containing an HO endonuclease cleavage site. Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.

Proper citation: RRID:Addgene_21670 Copy   

•   Source: Addgene

http://www.addgene.org/21674

Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:W. Kramer; Backbone Size:0; Vector Backbone:Ted; Vector Types:centromeric plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9343441
Comments: Derived from Ted, a centromeric plasmid (provided by W. Kramer) marked by the URA3 gene. Contains two copies of the Escherichia coli lacZ gene in inverted orientation, with one copy containing an HO endonuclease cleavage site. Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.

Proper citation: RRID:Addgene_21674 Copy   

•   Source: Addgene