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Vector Backbone Description: Backbone Size:5879; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for puromycin selection of transgenes in C. elegans and related species if transgene has a visual phenotype and Pmyo-2::mCherry expression is undesirable.
The unc-119 gene in pCG150 was replaced by the Prpl-28::PuroR::let-858_3'UTR cassette for puromycin selection in worms.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26301 Copy
Vector Backbone Description: Backbone Size:5969; Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for G418 selection of transgenes in C. elegans.
The PuroR gene in pBCN21-R4R3 was replaced by the NeoR gene for G418 resistance.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26304 Copy
Species: Homo sapiens
Genetic Insert: GAL4::p65
Vector Backbone Description: Backbone Size:8778; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26229 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9052; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Comments: Addgene NGS results identified a second GAL4 ORF in the plasmid sequence. This second ORF is not expected to affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_26226 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:9409; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26236 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:9409; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26235 Copy
Species: Homo sapiens
Genetic Insert: LexA::p65
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26231 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Zip-GAL4DBD
Vector Backbone Description: Backbone Size:8731; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26233 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Comments: extremely slow growing.
Proper citation: RRID:Addgene_26390 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26386 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:10104; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced in a Tet-On advanced cell line in order to obtain expression of the GOI.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.
Addgene found this plasmid to be extremely slow growing.
Proper citation: RRID:Addgene_26433 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:9185; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced in a Tet-On Advanced cell line for expression of the GOI.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.
Proper citation: RRID:Addgene_26432 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5070; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21344536
Proper citation: RRID:Addgene_26361 Copy
Vector Backbone Description: Backbone Size:10809; Vector Backbone:pCJF150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: The Prpl-28::PuroR::let-858_3'UTR cassette was inserted into the backbone of pCFJ150 to create a puromycin-selectable MosSCI vector for single copy integration in worms at the ttTi5605.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26346 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
Vector Backbone Description: Backbone Size:17037; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: Second Generation pie-1 vector for expressing ORFs in the germline
Proper citation: RRID:Addgene_26955 Copy
Genetic Insert: TALE Nuclease, NG for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32190 Copy
Genetic Insert: TALE Transcriptional Activator Backbone, NI for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32187 Copy
Genetic Insert: TALE Transcriptional Activator Backbone, NN for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32185 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.
Proper citation: RRID:Addgene_32318 Copy
Vector Backbone Description: Backbone Marker:Mo Bi Tec; Vector Backbone:pET01; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18930792
Proper citation: RRID:Addgene_32484 Copy
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