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Species: Saccharomyces Cerevisiae
Genetic Insert: Atg32
Vector Backbone Description: Vector Backbone:pET His6 TEV LIC cloning vector (1B); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29909755
Proper citation: RRID:Addgene_190431 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pFA6-yomRuby3-SpHIS5
Vector Backbone Description: Backbone Size:4021; Vector Backbone:pFA-Envy-SpHIS5 (Addgene plasmid 60782); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37436806
Proper citation: RRID:Addgene_204287 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tRNA-gRNA scaffold
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37326023
Comments: A common PCR template used fo CRISPR Engineering of EPisomes in Yeast, or CREEPY, a method for rapid engineering of large synthetic episomal DNA constructs. This is designed and assembled based on the template consisting of gRNA scaffold with a tRNA sequence, as published in Zhang, Yueping, et al. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae." Nature communications 10.1 (2019): 1053.
Proper citation: RRID:Addgene_200227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_192603 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_187970 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PMA1 promoter
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Backbone Size:2243; Vector Backbone:pICH41295; Vector Types:Synthetic Biology, Modular cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37575400
Proper citation: RRID:Addgene_200712 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MTF1
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31980825
Proper citation: RRID:Addgene_205067 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-Set2
Vector Backbone Description: Backbone Size:8173; Vector Backbone:hSyn-GW-IRES2-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34480866
Proper citation: RRID:Addgene_202089 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pRS426; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_187972 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MTF1
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31980825
Proper citation: RRID:Addgene_205069 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MTF1
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31980825
Proper citation: RRID:Addgene_205071 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MTF1
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31980825
Proper citation: RRID:Addgene_205070 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPO41
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19116203
Proper citation: RRID:Addgene_205072 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-Set2(R195G, C201A)
Vector Backbone Description: Backbone Size:8173; Vector Backbone:hSyn-GW-IRES2-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34480866
Proper citation: RRID:Addgene_202090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
Vector Backbone Description: Backbone Marker:Edgar Medina; Backbone Size:9378; Vector Backbone:pGI3EM17; Vector Types:Bacterial Expression, Yeast Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38236738
Comments: Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.
Proper citation: RRID:Addgene_210582 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BdTUBA1pro-Hshph-G4S-nanoluc-ScADH1ter
Vector Backbone Description: Backbone Marker:Edgar Medina; Backbone Size:9378; Vector Backbone:pGI3EM17; Vector Types:Bacterial Expression, Yeast Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38236738
Comments: Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.
Proper citation: RRID:Addgene_210586 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
Vector Backbone Description: Backbone Marker:Edgar Medina; Backbone Size:9375; Vector Backbone:pGI3EM30; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38236738
Comments: Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.
Proper citation: RRID:Addgene_210598 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
Vector Backbone Description: Backbone Marker:Edgar Medina; Backbone Size:9376; Vector Backbone:pGI3EM11; Vector Types:Bacterial Expression, Yeast Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38236738
Comments: Please visit https://doi.org/10.1101/2023.10.17.561934 for bioRxiv preprint.
Proper citation: RRID:Addgene_210569 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AGA1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212917 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SCW4
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212914 Copy
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