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Species: Saccharomyces cerevisiae
Genetic Insert: Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9
Vector Backbone Description: Vector Backbone:pUC118; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172645
Proper citation: RRID:Addgene_41562 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
Vector Backbone Description: Vector Backbone:pUC118; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172645
Proper citation: RRID:Addgene_41561 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9
Vector Backbone Description: Vector Backbone:pUC118; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172645
Proper citation: RRID:Addgene_41560 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41599 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41597 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41595 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41614 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1 promoter
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717241
Proper citation: RRID:Addgene_41611 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SEC63-mRFP
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18812321
Comments: Please note that Addgene's quality control sequence shows several discrepancies with depositor's reference sequence. These are in vector region and should not affect function.
Proper citation: RRID:Addgene_41842 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AVT1
Vector Backbone Description: Backbone Marker:Dan Gottschling (Addgene plasmid# 41894); Backbone Size:7882; Vector Backbone:pAG306-GPD-ccdB chr I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172144
Proper citation: RRID:Addgene_41898 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mediator of the Replication Checkpoint
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6021; Vector Backbone:pRS415; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12865299
Comments: There is a D304N mutation in LEU, relative to aa1 in LEU, it is not thought to affect function.
Proper citation: RRID:Addgene_41924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39337 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39336 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39338 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LEU2
Vector Backbone Description: Backbone Marker:ATCC, Number: 77142; Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23222782
Proper citation: RRID:Addgene_40276 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS4
Vector Backbone Description: Backbone Size:5037; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21495 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SNF7
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21492 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS20
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21490 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: polyA polymerase
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5400; Vector Backbone:Pet21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15909992
Comments: See attached purification scheme
Proper citation: RRID:Addgene_21716 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ura3
Vector Backbone Description: Backbone Marker:R. Kostriken; Backbone Size:2700; Vector Backbone:pRK113; Vector Types:integration sequence; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3065627
Comments: Constructed by inserting a HindIII URA3 fragment into the HindIII
site of the multiple cloning site of plasmid pRK113.
Contains the 117-bp HO cut site of
MATa inserted between the HincII and BamHI sites of pUC9 and the URA3 1.17-kb HindIII fragment at the HindlIl
site transcribed away from the HO cut site.
Proper citation: RRID:Addgene_21677 Copy
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