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Species: Chloramphenicol resistance gene (CmR) - ccdB gene
Genetic Insert: Chloramphenicol resistance gene (CmR) - ccdB gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9956; Vector Backbone:pLenti6/UbC/V5-Dest; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20132838
Comments: V5 tag deleted
Proper citation: RRID:Addgene_24178 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The mouse PGK promoter was PCR-amplified and cloned into pLD-puro-CnVA (Plasmid #24587) to generate pLD-puro-PGKnVA.
VA= versatile affinity tag, 3XFlag, 6XHis+StrepIII
Proper citation: RRID:Addgene_24589 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EnVA using NdeI/NheI to generate pLD-puro-CnVA.
pLD-puro-EnVA (pLD-puromycin resistance- EF1alpha, N-terminal VA tag) was generated as described in the paper from pLKO.1
VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII
Proper citation: RRID:Addgene_24587 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: VA= Versatile Affinity tag, 3XFlag+6XHis+StrepIII
Proper citation: RRID:Addgene_24592 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: pLD-hygro-EnVM was constructed by subcloning the hygromycin resistance gene from the pLJM6 vector (J Moffat, unpublished) into the pLD-puro-EnFH vector using SpeI/NsiI and subsequently subcloning a gene synthesized V5-Myc (VM) tag (Bio Basic Inc.) from the pUC57 host vector.
pLD-puro-EnFH (pLD-puromycin resistance- EF1alpha, N-terminus triple Flag-6-His) was generated from pLKO.1 (see paper for details).
Proper citation: RRID:Addgene_24590 Copy
Species: reporter gene mCherry
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10462; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24567 Copy
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:13918; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16945906
Comments: Parent lentivector, co-expression of Hygromycin selection gene
Proper citation: RRID:Addgene_25737 Copy
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:13286; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16945906
Comments: Parent lentivector, co-expression of Neomycin selection gene
Proper citation: RRID:Addgene_25735 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:16320; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16499902
Comments: Use ONLY DB3.1 to transform this plasmid. DH5 alpha does not work (it has a death gene).
Proper citation: RRID:Addgene_22720 Copy
Species: artifical
Genetic Insert: Gateway-SnAvi
Vector Backbone Description: Backbone Marker:pTriEx5 (Novagen); Backbone Size:6027; Vector Backbone:pBY2887; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20047968
Proper citation: RRID:Addgene_23222 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25916 Copy
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21706014
Comments: There is a Stop codon directly after the Gateway sequence.
Proper citation: RRID:Addgene_25895 Copy
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21706014
Comments: There is a Stop codon directly after the Gateway sequence.
Proper citation: RRID:Addgene_25897 Copy
Vector Backbone Description: Backbone Size:9573; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21706014
Comments: There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.
Proper citation: RRID:Addgene_25896 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25947 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25942 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25944 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20515414
Proper citation: RRID:Addgene_25961 Copy
Genetic Insert: Tev Protease Cleavage site
Vector Backbone Description: Backbone Size:2720; Vector Backbone:pMLL-AC; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Note that this plasmid must be grown in both Ampicillin and Chloramphenicol
Proper citation: RRID:Addgene_26186 Copy
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for phleomycin selection of transgenes in C. elegans.
The PuroR gene in pBCN21-R4R3 was replaced by the ZeocinR gene for phleomycin resistance.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26305 Copy
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