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Species: Saccharomyces cerevisiae
Genetic Insert: S. cerevisiae TEF2 promoter
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pFA6a–link–yEGFP–Kan; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33311538
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.11.089300v1 for bioRxiv preprint.
Parent vector is described in "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae", Mark A Sheff , Kurt S ThornYeast 2004 Jun;21(8):661-70. doi: 10.1002/yea.1130. The parent vector was intended for GFP tagging of yeast proteins. We placed the TEF2 promoter directly upstream of GFP for sugar-insensitive expression of GFP under galactose or dextrose conditions.
Proper citation: RRID:Addgene_160432 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153106 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153101 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: α Galactosidase SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153504 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Invertase 2 SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153493 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Prepro SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: membrane localization signal-tagBFP-wrmScarlet11
Vector Backbone Description: Backbone Size:5350; Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33693628
Proper citation: RRID:Addgene_158585 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADR1c
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158600 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ARG3pr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164709 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GALLpr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: This plasmid was made by Catherine Oikonomou in Dr. Cross's lab.
yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164702 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP1pr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164703 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1pr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164707 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tetOpr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37369667
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164705 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P(Z)-mcherry
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pNTI661; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33303588
Proper citation: RRID:Addgene_164918 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HO endonuclease
Vector Backbone Description: Backbone Marker:Addgene plasmid # 64536; Backbone Size:4434; Vector Backbone:pRG206MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33053188
Comments: Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
Proper citation: RRID:Addgene_154821 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP2
Vector Backbone Description: Backbone Marker:self-made; derived from the BioBrick assembly plasmid pSB1C3 ; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160599 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CIM5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16810255
Proper citation: RRID:Addgene_16074 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNA Polymerase II CTD
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468530
Proper citation: RRID:Addgene_160688 Copy
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