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Species: Saccharomyces cerevisiae
Genetic Insert: RPL25
Vector Backbone Description: Backbone Size:5000; Vector Backbone:yEGFP3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Entire RPL25 ORF inserted upstream of eGFP in EcoRI/HindIII of yEGFP3
Proper citation: RRID:Addgene_24037 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rRNA 5'ETS ntds 923 to 1179
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23995 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 25S rRNA from +5270 to oligo y
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23996 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rDNA 5'ETS ntds 1 to 177
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23993 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KAP123
Vector Backbone Description: Backbone Marker:Rosemary Williams (Rout Lab); Backbone Size:7100; Vector Backbone:pYEX-U (modified pYEX-BX); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: KAP123 fused upstream of two FLU tags in BamHI/SalI of pBT024
Proper citation: RRID:Addgene_24048 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rRNA 5'ETS ntds 347 to 631
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pTOPO; Vector Types:Bacterial Cloning - TOPO; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15489292
Proper citation: RRID:Addgene_23994 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NAB2
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pYX242; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NLS of NAB2 (bp 601-750) inserted in EcoRI/BamHI, eGFP inserted downstream in BamHI/HindIII and a single PrA repeat inserted further downstream in HindIII/SalI of pYX242.
PrA motif sequence downstream of GFP:
GCTCAACAAAATGCTTTTTATCAAGTCTTAAATATGCCTAACTTAAATGCTGATCAACGCAATGGTTTTATCCAAAGCCTTAAAGATGATCCAAGCCAAAGTGCTAACGTTTTAGGTGAAGCTCAAAAACTTAATGACTCTCAAGCTCCAAAAGCTGATGCGCAACAAAATAAC
Proper citation: RRID:Addgene_24043 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PHO4
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pYX242; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NLS of PHO4 (bp 421-588) inserted in EcoRI/BamHI, eGFP inserted downstream in BamHI/HindIII and a single PrA repeat inserted further downstream in HindIII/SalI of pYX242.
PrA motif sequence downstream of GFP:
GCTCAACAAAATGCTTTTTATCAAGTCTTAAATATGCCTAACTTAAATGCTGATCAACGCAATGGTTTTATCCAAAGCCTTAAAGATGATCCAAGCCAAAGTGCTAACGTTTTAGGTGAAGCTCAAAAACTTAATGACTCTCAAGCTCCAAAAGCTGATGCGCAACAAAATAAC
Proper citation: RRID:Addgene_24044 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KAP95
Vector Backbone Description: Backbone Marker:Rosemary Williams (Rout Lab); Backbone Size:7100; Vector Backbone:pYEX-U (modified pYEX-BX); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: KAP95 fused upstream of two FLU tags in BamHI/SalI of pBT024
Proper citation: RRID:Addgene_24045 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20017217
Proper citation: RRID:Addgene_24585 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20017217
Comments: The TEF1 (S.C.) promoter region has 4 base differences from the published sequences but the authors report that they do not affect transcriptional function.
Proper citation: RRID:Addgene_24584 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FAA2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBR322; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20111002
Proper citation: RRID:Addgene_24631 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: REV1
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19528320
Comments: The REV1 ORF plus 1050 nucleotides (nt) of its upstream region that include the REV1 promoter and 500nt of the downstream region that include the REV1 transcription terminator was cloned in the vector YCplac111, which carries the LEU2 gene, the autonomous replicating sequence ARS1, and the centromeric CEN4 region.
A BglII restriction site was added by site-directed mutagenesis right before the stop codon of REV1. A 33-mer oligonucleotide duplex containing the HA-coding sequence and a BamHI and a BglII restriction site was inserted into the BglII restriction site, adding an HA tag in fusion with REV1, and regenerating a unique BglII restriction site. This step was repeated three times in order to get four HA tags in fusion with REV1.
Proper citation: RRID:Addgene_22287 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Comments: Mutation abolishes the ubiquitin ligase function
Proper citation: RRID:Addgene_22288 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Proper citation: RRID:Addgene_22289 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23056 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23064 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23068 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III - Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23065 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23066 Copy
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