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Species: Caenorhabditis elegans
Genetic Insert: rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
Vector Backbone Description: Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Comments: Several differences are present in the Addgene NGS sequence compared to the theoretical sequence present in the attached Genbank file that do not impact the function of the plasmid.
Proper citation: RRID:Addgene_122629 Copy
Species: Caenorhabditis elegans
Genetic Insert: CFP
Vector Backbone Description: Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_122621 Copy
Species: Caenorhabditis elegans
Genetic Insert: Zip(-)::QFAD::NLS::SL2::mCherry
Vector Backbone Description: Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Comments: Several differences are present in the Addgene NGS sequence compared to the theoretical sequence present in the attached Genbank file that do not impact the function of the plasmid.
Proper citation: RRID:Addgene_122615 Copy
Species: Caenorhabditis elegans
Genetic Insert: SV40 NLS::rtetR::Zip(+)::SL2::mCherry
Vector Backbone Description: Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Comments: Several differences are present in the Addgene NGS sequence compared to the theoretical sequence present in the attached Genbank file that do not impact the function of the plasmid.
Proper citation: RRID:Addgene_122616 Copy
Species: Caenorhabditis elegans
Genetic Insert: 7XTRE-delta-pes-10-GFP
Vector Backbone Description: Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_122613 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004).
Alternate plasmid name: pIC26 + MEX-5 (Dendra + MEX-5 ORF)
Proper citation: RRID:Addgene_40077 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5
Proper citation: RRID:Addgene_40074 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, R183-5A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 R183-5A
Proper citation: RRID:Addgene_40150 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, wild-type
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 wild-type
Proper citation: RRID:Addgene_40149 Copy
Species: Caenorhabditis elegans
Genetic Insert: gfp::par-2 RNAi resistant R183-5A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-gfp::par-2 R183-5A
Proper citation: RRID:Addgene_40148 Copy
Species: Caenorhabditis elegans
Genetic Insert: gfp::par-2 wild-type RNAi resistant
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-gfp::par-2 wild-type
Proper citation: RRID:Addgene_40147 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (S241A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR S241A
Proper citation: RRID:Addgene_40144 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R183-5A + S241A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R183-5A + S241A
Proper citation: RRID:Addgene_40142 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R163A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R163A
Proper citation: RRID:Addgene_40140 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (K162A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR K162A
Proper citation: RRID:Addgene_40139 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A, S458A)
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A, S458A)
Proper citation: RRID:Addgene_40089 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21925318
Comments: A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene.
Alternate plasmid name: pDONR P2RP3+MEX-5 genomic(RNAI res exon 2) + 3’UTR
Proper citation: RRID:Addgene_40122 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A)
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A)
Proper citation: RRID:Addgene_40088 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR(S404A) - mex-5 3'UTR
Proper citation: RRID:Addgene_40192 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR
Proper citation: RRID:Addgene_40191 Copy
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