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Vector Backbone Description: Backbone Marker:Lorenz et al, 1996 (PubMed ID: 8917596); Backbone Size:5246; Vector Backbone:pTLN; Vector Types:Xenopus oocytes expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Iwan Zimmermann, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49032 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5855; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49030 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6578; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49029 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5862; Vector Backbone:pGL4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25177895
Comments: Please note that the plasmid sequence contains an IS1 element, and the total size of the plasmid appears to be 7.5-8kb. This difference does not affect the function of the plasmid as described in the publication.
Proper citation: RRID:Addgene_49350 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49631 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49630 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49624 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49626 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24324157
Comments: This destination vector for TALEs is compatible with the Zhang Lab TALE Toolbox.
Proper citation: RRID:Addgene_49625 Copy
Species: Chloramphenicol resistance gene (CmR) - ccdB gene
Genetic Insert: Chloramphenicol resistance gene (CmR) - ccdB gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9956; Vector Backbone:pLenti6/UbC/V5-Dest; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20132838
Comments: V5 tag deleted
Proper citation: RRID:Addgene_24178 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The mouse PGK promoter was PCR-amplified and cloned into pLD-puro-CnVA (Plasmid #24587) to generate pLD-puro-PGKnVA.
VA= versatile affinity tag, 3XFlag, 6XHis+StrepIII
Proper citation: RRID:Addgene_24589 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EnVA using NdeI/NheI to generate pLD-puro-CnVA.
pLD-puro-EnVA (pLD-puromycin resistance- EF1alpha, N-terminal VA tag) was generated as described in the paper from pLKO.1
VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII
Proper citation: RRID:Addgene_24587 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: VA= Versatile Affinity tag, 3XFlag+6XHis+StrepIII
Proper citation: RRID:Addgene_24592 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: pLD-hygro-EnVM was constructed by subcloning the hygromycin resistance gene from the pLJM6 vector (J Moffat, unpublished) into the pLD-puro-EnFH vector using SpeI/NsiI and subsequently subcloning a gene synthesized V5-Myc (VM) tag (Bio Basic Inc.) from the pUC57 host vector.
pLD-puro-EnFH (pLD-puromycin resistance- EF1alpha, N-terminus triple Flag-6-His) was generated from pLKO.1 (see paper for details).
Proper citation: RRID:Addgene_24590 Copy
Species: reporter gene mCherry
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10462; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24567 Copy
Species: Synthetic
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pGP8GF; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32123041
Proper citation: RRID:Addgene_188445 Copy
Vector Backbone Description: Vector Backbone:pCFJ1258; Vector Types:Multisite Gateway [4-3] Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188885 Copy
Vector Backbone Description: Vector Backbone:pCF150; Vector Types:MosSci targeting vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34748534
Proper citation: RRID:Addgene_188893 Copy
Vector Backbone Description: Vector Backbone:pMOD_B0000; Vector Types:cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176874 Copy
Vector Backbone Description: Vector Backbone:pMOD_C'0000a; Vector Types:cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176875 Copy
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