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Species: Saccharomyces cerevisiae
Genetic Insert: Glo3 S389,398 A
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_129485 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Glo3 S389,398 D
Vector Backbone Description: Vector Backbone:P415 Met25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31331965
Proper citation: RRID:Addgene_129486 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP70 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19516 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBluescript SK+; Vector Types:carries FLP recombinase cDNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CFP-FKBP-Inp54p (D281A)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16990515
Proper citation: RRID:Addgene_20156 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO-conjugating enzyme Ubc9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_20368 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16980971
Proper citation: RRID:Addgene_25464 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Synthetic Green Fluorescent Protein - VRG4
Vector Backbone Description: Backbone Size:5500; Vector Backbone:YCPlac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16699524
Proper citation: RRID:Addgene_25447 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sec13-Green Fluorescent Protein
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pUC19-URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10189369
Proper citation: RRID:Addgene_25445 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Med7 aa102-205 for C-terminal tagging
Vector Backbone Description: Backbone Size:2852; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26184 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Med21 aa1-132 for N-terminal tagging
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26183 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Med21 aa1-132 for C-terminal tagging
Vector Backbone Description: Backbone Size:2852; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26185 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Proper citation: RRID:Addgene_26265 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ZIP- Gal4DBD
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Comments: Luan et al. Neuron. 2006 Nov 9. 52(3):425-36.
Proper citation: RRID:Addgene_26264 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4AD-Zip+
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Comments: Luan et al. Neuron. 2006 Nov 9. 52(3):425-36.
Proper citation: RRID:Addgene_26263 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4::VP16
Vector Backbone Description: Backbone Size:8770; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26228 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9223; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26227 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: nlsLexA::GADfl
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26232 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-GLuc
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182213 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-Akaluc
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, TEM imaging; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182212 Copy
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