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Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191623 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191622 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191628 Copy
Species: Pseudomonas putida F1
Genetic Insert: CymR
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_192273 Copy
Species: Eggerthella lenta
Genetic Insert: Dopamine dehydroxylase (R506S mutation, inactive form)
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191631 Copy
Species: Pseudomonas putida F1
Genetic Insert: CymR
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191634 Copy
Species: Pseudomonas putida F1
Genetic Insert: CymR
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191633 Copy
Species: Eggerthella lenta
Genetic Insert: Dopamine dehydroxylase
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191632 Copy
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191247 Copy
Genetic Insert: the groESL region region from E. coli is the insert in the pBR325 plasmid pOF39
Vector Backbone Description: Vector Backbone:the pOF39 plasmid backbone is pBR325; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36973666
Comments: Please see the BM28 genome sequence (https://www.ncbi.nlm.nih.gov/nuccore/CP102380) and the accompanying papers for more details.
Additional publications:
Rudolph B, Gebendorfer KM, Buchner J, Winter J. Evolution of Escherichia coli for growth at high temperatures. J Biol Chem. 2010 Jun 18;285(25):19029-34. doi: 10.1074/jbc.M110.103374. Epub 2010 Apr 20. PMID: 20406805; PMCID: PMC2885180.
And for the pOF39 plasmid: Fayet O, Louarn JM, Georgopoulos C. Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes. Mol Gen Genet. 1986 Mar;202(3):435-45. doi: 10.1007/BF00333274. PMID: 3012269.
Proper citation: RRID:Addgene_201047 Copy
Species: Synthetic
Genetic Insert: tdMCP-12xGCN4
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36867026
Proper citation: RRID:Addgene_205158 Copy
Species: Synthetic
Genetic Insert: tdMCP-msfGFP(V206K)-GBI
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36867026
Proper citation: RRID:Addgene_205163 Copy
Genetic Insert: Sh-CAST sgRNA 2300
Vector Backbone Description: Backbone Size:4812; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170984 Copy
Genetic Insert: mRFP
Vector Backbone Description: Backbone Size:4879; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170982 Copy
Species: Homo sapiens
Genetic Insert: trLAT
Vector Backbone Description: Backbone Marker:Ilya Levental lab; Vector Backbone:trLAT-mRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203745 Copy
Species: Homo sapiens
Genetic Insert: 2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
Vector Backbone Description: Vector Backbone:AAVS1-SA-Puro; Vector Types:ZNF donor; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36989136
Comments: For this purpose, a previously generated backbone containing 2xCHS4-EF1a-tdTomato-SV40-2xCHS4 was used (Bagley et al., 2017).
Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_196192 Copy
Species: Synthetic
Genetic Insert: PAGFP-FLAG-Kan
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_211834 Copy
Species: Synthetic
Genetic Insert: sfGFP-FLAG-Kan
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_211833 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:PCR4-TOPO; Vector Types:Mammalian Expression, Mouse Targeting, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:39762235
Proper citation: RRID:Addgene_211940 Copy
Genetic Insert: Yellow fluorescent protein
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pMB1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:33443634
Proper citation: RRID:Addgene_215654 Copy
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