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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pTH398-SUP45-G54D
 
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RRID:Addgene_29376 SUP45 gene encoding translation release factor 1 from S. cerevisiae Saccharomyces cerevisiae Ampicillin PMID:20444877 Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin G54D mutant gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon. 2026-09-05 12:54:52 0
pTH394-SUP45-L34S
 
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RRID:Addgene_29373 SUP45 gene encoding translation release factor 1 from S. cerevisiae Saccharomyces cerevisiae Ampicillin PMID:20444877 Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin L34S mutant gene (corresponding to the sup45-36ts allele) including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon. 2026-09-05 12:54:52 0
pTH363-SUP45-F401Y
 
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RRID:Addgene_29394 SUP45 gene encoding translation release factor 1 from S. cerevisiae Saccharomyces cerevisiae Ampicillin PMID:20444877 Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin F401Y mutant gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon. 2026-09-05 12:54:52 0
pTH397-SUP45-I222S
 
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1+ mentions
RRID:Addgene_29388 SUP45 gene encoding translation release factor 1 from S. cerevisiae Saccharomyces cerevisiae Ampicillin PMID:20444877 Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin I222S mutant gene (corresponding to the sup45-2 allele) including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon. 2026-09-05 12:54:52 1
pTH444-SUP45-G181A
 
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RRID:Addgene_29387 SUP45 gene encoding translation release factor 1 from S. cerevisiae Saccharomyces cerevisiae Ampicillin PMID:20444877 Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin G181A mutant gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon. 2026-09-05 12:54:52 0
pTH441-SUP45-K175T
 
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RRID:Addgene_29384 SUP45 gene encoding translation release factor 1 from S. cerevisiae Saccharomyces cerevisiae Ampicillin PMID:20444877 Backbone Size:5976; Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin K175T mutant gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon. 2026-09-05 12:54:52 0
pBacPAK8-His-myc-Uba1
 
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RRID:Addgene_29508 Uba1 Saccharomyces cerevisiae Ampicillin PMID:10973499 Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:54:53 0
pDS221
 
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1+ mentions
RRID:Addgene_34981 ePDZb1–mCherry Saccharomyces cerevisiae Ampicillin PMID:22388287 Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:55:27 4
pDS220
 
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1+ mentions
RRID:Addgene_34980 ePDZb–mCherry Saccharomyces cerevisiae Ampicillin PMID:22388287 Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:55:27 1
pDS295
 
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RRID:Addgene_34984 ePDZb Saccharomyces cerevisiae Ampicillin PMID:22388287 Vector Backbone:YIPlac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:55:27 0
pDS257
 
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1+ mentions
RRID:Addgene_34971 Mid2(SS/TM)-GFP-LOVpep Saccharomyces cerevisiae Ampicillin PMID:22388287 The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details. Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:55:27 1
pDS255
 
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RRID:Addgene_34970 Mid2(SS/TM)-GFP-LOVpep Saccharomyces cerevisiae Ampicillin PMID:22388287 The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details. Backbone Size:4263; Vector Backbone:YIPlac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin T406A, T407A 2026-09-05 12:55:27 0
pDS191
 
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RRID:Addgene_34978 ePDZb Saccharomyces cerevisiae Ampicillin PMID:22388287 Backbone Marker:EUROSCARF; Backbone Size:6624; Vector Backbone:pGREG503; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:55:27 0
pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
 
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1+ mentions
RRID:Addgene_35152 AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA Saccharomyces cerevisiae Chloramphenicol PMID:21952217 Please note that the R364 in Mevalonate Kinase (MK) may be susceptible to mutations. Although the original plasmid encoded R364C, Addgene's stock contains R364S. This mutation is not expected to impact overall function, however the exact effects are unknown. Vector Backbone:pBbA5c; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol R364S in Mevalonate Kinase 2026-09-05 12:55:29 3
pSA48
 
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RRID:Addgene_35112 lys5MX3 Saccharomyces cerevisiae Ampicillin PMID:14745782 Vector Backbone:pFA6-kanMX4; Vector Types:Yeast Replicating; Bacterial Resistance:Ampicillin 2026-09-05 12:55:28 0
pSA25
 
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RRID:Addgene_35116 lys5MX4 Saccharomyces cerevisiae Ampicillin PMID:14745782 Vector Backbone:pRS316; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin 2026-09-05 12:55:28 0
pBN63
 
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1+ mentions
RRID:Addgene_35104 fcy1PR Saccharomyces cerevisiae Ampicillin PMID:16088873 Vector Backbone:pFA6-kanMX3; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin 2026-09-05 12:55:28 1
pPH37
 
Resource Report
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1+ mentions
RRID:Addgene_35102 fcy1MX4 Saccharomyces cerevisiae Ampicillin PMID:16088873 Vector Backbone:pFA6-kanMX4; Vector Types:Yeast replicating; Bacterial Resistance:Ampicillin 2026-09-05 12:55:28 2
pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
 
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RRID:Addgene_35191 loxP KAN loxP 24xPP7SL MDN1 Saccharomyces cerevisiae Ampicillin PMID:21512033 This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid. Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert. Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function. Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2026-09-05 12:55:29 0
pRS424GFP-2xPH(Osh2)
 
Resource Report
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1+ mentions
RRID:Addgene_36095 2xPH(Osh2) fusion Saccharomyces cerevisiae Ampicillin PMID:21295699 Please note that the first Osh2 domain contains aa273-421 and the second Osh2 domains contains aa260-421 when compared to GenBank reference sequence NP_010265.1. According to this reference sequence the PH domain is aa 292–384. The additional residues surrounding the PH domain were intentionally cloned into this construct. Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:55:36 2

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